|
| Status |
Public on Apr 26, 2012 |
| Title |
Genome-wide mapping of ligand-dependent progesterone receptor chromatin interactions in human breast cell lines using PR ChIP-seq |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The effects of progesterone are pleiotropic, resulting in diverse outcomes in a range of target tissues. Progesterone signalling is mediated through the nuclear progesterone receptor (PR) and to identify whether the cell specificity of the PR transcriptome arises from distinct patterns of genomic interaction of PR, we have mapped the genomic binding sites for PR in breast cancer cells and minimally transformed breast cells. PR binding was correlated with transcriptional outcome in both cell lines.
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| |
|
| Overall design |
Three replicate PR ChIP samples and two replicate input DNA control samples from T-47D breast cancer cells and two replicate PR ChIP samples and two replicate input DNA control samples from AB32 transformed normal breast cells.
|
| |
|
| Contributor(s) |
Graham JD, Clarke CL |
| Citation(s) |
22545144 |
| |
| Submission date |
Aug 02, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
J Dinny Graham |
| E-mail(s) |
dinny.graham@sydney.edu.au
|
| Phone |
61 2 86273702
|
| Organization name |
University of Sydney
|
| Department |
Westmead Institute for Medical Research
|
| Lab |
Translational Breast Cancer Genomics
|
| Street address |
176 Hawkesbury Rd
|
| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (9)
|
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| This SubSeries is part of SuperSeries: |
| GSE31130 |
Non-overlapping progesterone receptor cistromes contribute to cell-specific transcriptional outcomes in breast cells |
|
| Relations |
| SRA |
SRP007830 |
| BioProject |
PRJNA154657 |
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