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| Status |
Public on Jun 20, 2012 |
| Title |
MicroRNA expression profiling of Ewing sarcoma spheres vs. adherent cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We have recently demonstrated that human paediatric mesenchymal stem cells can be reprogrammed toward a Ewing’s sarcoma family tumor (ESFT) cancer stem cell (CSC) phenotype by mechanisms that implicate microRNAs (miRNAs). Here, we show that the miRNA profile of ESFT CSC is shared by embryonic stem cells and CSC from divergent tumor types. We also provide evidence that the miRNA profile of ESFT CSC is the result of reversible disruption of TARBP2-dependent miRNA maturation. Restoration of TARBP2 activity and systemic delivery of synthetic forms of either of two of its targets, miRNA-143 or miRNA-145, inhibited ESFT CSC clonogenicity and tumor growth in vivo. Our observations suggest that CSC self-renewal and tumor initiation may depend on deregulation of TARBP2-dependent miRNA expression.
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| Overall design |
2 Ewing sarcoma primary samples: comparison between spheres and derived adherent cells.
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| Contributor(s) |
De Vito C, Riggi N, Suvà M, Baumer K, Provero P, Stamenkovic I |
| Citation(s) |
22698405 |
| Submission date |
Aug 02, 2011 |
| Last update date |
Jun 22, 2012 |
| Contact name |
Paolo Provero |
| E-mail(s) |
paolo.provero@unito.it
|
| Organization name |
University of Turin
|
| Department |
Molecular Biotechnology and Health Sciences
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| ZIP/Postal code |
I-10100 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL11432 |
miRCURY LNA microRNA Array, 5th generation - hsa, mmu & rno |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE31146 |
A TARBP2-dependent miRNA expression profile determines cancer stem cell properties and provides new candidate therapeutic reagents in Ewing's sarcoma |
|
| Relations |
| BioProject |
PRJNA154091 |
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