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| Status |
Public on Aug 07, 2012 |
| Title |
Benzo[a]pyrene-induced changes in microRNA-mRNA networks |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The microarray contains 9360 reporters, each present four times on the microarray. In our analysis 1326 human and viral reporters were used, of which 880 are unique human mature microRNAs. The other 8024 reporters represent microRNAs from other species. cDNA was generated using 1 μg of total RNA per sample. RNA was labeled only with Hy3 containing dye using the microRNACURY LNA™ microRNA Hy3 Power labeling kit (Exiqon, Denmark) according to the manufacturer’s protocol in a total volume of 25 μl. To adjust the volume to 100 μl, a hybridization buffer provided in miRCURY LNA™ microRNA Array, 5th generation kit (Exiqon, Denmark) was used.
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| Overall design |
For each biological experiment, one hybridization per time point was conducted and one sample per array. In total, 24 arrays were used for 8 different conditions (BaP or control, at 6, 12, 24 and 48 hours).
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| Contributor(s) |
Lizarraga D, Gaj S, Brauers K, Kleinjans JC, van Delft JH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 08, 2011 |
| Last update date |
Aug 08, 2012 |
| Contact name |
Daneida Lizarraga |
| E-mail(s) |
D.Lizarraga@maastrichtuniversity.nl
|
| Fax |
+31 43 3884146
|
| URL |
http://www.toxicogenomics-um.nl/Contact
|
| Organization name |
Maastricht University
|
| Department |
Toxicogenomics
|
| Street address |
Universiteitssingel 50
|
| City |
Maastricht |
| State/province |
Limburg |
| ZIP/Postal code |
6229ER |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11432 |
miRCURY LNA microRNA Array, 5th generation - hsa, mmu & rno |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA146025 |
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