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| Status |
Public on Nov 16, 2011 |
| Title |
Gene expression profile of preclinical arthritis and its modulation by antigen-induced tolerance |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
During the course of adjuvant arthritis, maximal changes in gene expression were observed at the incubation phase. A major group of genes affected was related to immune activity. Tolerance induction by mycobacterial heat-shock protein 65 (Bhsp65), the disease-related antigen, caused upregulation of a large number of genes. These included immune activity genes as well as cell proliferation-related genes.
We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process.
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| Overall design |
The draining lymph node cells (LNC) were harvested from arthritic rats at different phases of adjuvant arthritis (incubation,peak and recovery). Also tested were LNC of naïve rats. LNC were tested ex vivo. Total RNA isolated from LNC was used for testing with affymetrix gene chip following manufacturer's instructions. Similarly, LNC of Bhsp65-tolerized rats harvested at incubation phase were tested with or without Bhsp65 restimulation in vitro. LNC of arthritic rats in incubation phase were similarly restimulated with Bhsp65 in vitro as controls, and the results compared with that of the tolerized rats.
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| Contributor(s) |
Yu H, Lu C, Tan M, Moudgil KD |
| Citation(s) |
21914168 |
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| Submission date |
Aug 10, 2011 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Kamal D. Moudgil |
| E-mail(s) |
kmoud001@umaryland.edu
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| Phone |
410-706-7804
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| Fax |
410-706-2129
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| Organization name |
University of Maryland School of Medicine
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| Department |
Microbiology and Immunology
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| Street address |
685 W. Baltimore Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM776502 |
LNC Incubation, biological rep1 |
| GSM776503 |
LNC Incubation, biological rep2 |
| GSM776504 |
LNC Incubation, biological rep3 |
| GSM776505 |
LNC Peak, biological rep1 |
| GSM776506 |
LNC Peak, biological rep2 |
| GSM776507 |
LNC Peak, biological rep3 |
| GSM776508 |
LNC Recovery, biological rep1 |
| GSM776509 |
LNC Recovery, biological rep2 |
| GSM776510 |
LNC Recovery, biological rep3 |
| GSM776511 |
LNC Pre-arthritic medium, biological rep1 |
| GSM776512 |
LNC Pre-arthritic medium, biological rep2 |
| GSM776513 |
LNC Pre-arthritic medium, biological rep3 |
| GSM776514 |
LNC Pre-arthritic Bhsp65, biological rep1 |
| GSM776515 |
LNC Pre-arthritic Bhsp65, biological rep2 |
| GSM776516 |
LNC Pre-arthritic Bhsp65, biological rep3 |
| GSM776517 |
LNC Bhsp65-tolerized, medium, biological rep1 |
| GSM776518 |
LNC Bhsp65-tolerized, medium, biological rep2 |
| GSM776519 |
LNC Bhsp65-tolerized, medium, biological rep3 |
| GSM776520 |
LNC Bhsp65-tolerized, Bhsp65, biological rep1 |
| GSM776521 |
LNC Bhsp65-tolerized, Bhsp65, biological rep2 |
| GSM776522 |
LNC Bhsp65-tolerized, Bhsp65, biological rep3 |
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| Relations |
| BioProject |
PRJNA146083 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31314_RAW.tar |
94.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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