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| Status |
Public on Aug 17, 2014 |
| Title |
Differential expression analysis between litR (TTHB100) deletion mutant and wild-type of Thermus thermophilus HB8 under the dark condition. |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
We observed the expression profile of the total mRNA of litR (TTHB100) deletion mutant of T. thermophilus HB8 strain grown in a rich (TM) medium at 60 °C under the dark condition compared with that of wild-type.
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| Overall design |
Three wild-type strain of T. thermophilus HB8 were pre-cultured at 60 °C for 16 hours in 100 mL of TM medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.5 with NaOH. The cells (1 mL) were inoculated into 100 mL of the synthetic medium and then cultivated at 60°C for 8 hours under dark condition. Then the 1.0 mM of ZnSO4 was added into the medium, and continued cultivation. Cells were collected, and then, the expression of each mRNA was analyzed.
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| Contributor(s) |
Takano H, Agari Y, Shinkai A, Ueda K, Kuramitsu S |
| Citation(s) |
25294106 |
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| Submission date |
Aug 18, 2011 |
| Last update date |
Jan 07, 2015 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
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| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA145777 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31463_RAW.tar |
8.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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