|
| Status |
Public on Apr 08, 2012 |
| Title |
Sex-biased gene expression in Drosophila melanogaster larvae |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Genes with sex-biased expression in adults experience unique evolutionary dynamics. It is unclear, however, whether the selection pressures responsible for these well documented patterns also act upon genes with sex-biased expression in other developmental stages. To examine this, we measured expression in male and female Drosophila melanogaster larvae.
|
| |
|
| Overall design |
Drosophila melanogaster wandering third instar larvae were sexed using the visible gonad. RNA was isolated from three replicate samples of male and female larvae and one sample each of adult males and females. RNA was prepared following the manufacturer's instructions, using single color labelling. Each sample/replicate was hybridized to one sector of the Agilent 4 sector array (a total of two arrays were used), with the following design: Array 1 had one larval male sample, one larval female sample, one adult male sample, and one adult female sample; Array 2 had two larval male samples and two larval female samples.
|
| |
|
| Contributor(s) |
Meisel RP, Clark AG |
| Citation(s) |
22499666 |
| Submission date |
Aug 29, 2011 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Richard Meisel |
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77204-5001 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6385 |
Agilent-018972 D. melanogaster (FruitFly) Oligo Microarray 4x44K G2519F (Feature Number version) |
|
| Samples (8)
|
| GSM787629 |
Drosophila melanogaster larval female rep1 |
| GSM787630 |
Drosophila melanogaster larval male rep1 |
| GSM787631 |
Drosophila melanogaster adult female rep1 |
| GSM787632 |
Drosophila melanogaster adult male rep1 |
| GSM787633 |
Drosophila melanogaster larval female rep2 |
| GSM787634 |
Drosophila melanogaster larval male rep2 |
| GSM787635 |
Drosophila melanogaster larval female rep3 |
| GSM787636 |
Drosophila melanogaster larval male rep3 |
|
| Relations |
| BioProject |
PRJNA145347 |
Less...
More...