|
| Status |
Public on Apr 01, 2012 |
| Title |
Regulation of gene expression by mir-493 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify genes that are inhibited by mir-493, we have employed whole genome microarray expression profiling as a discovery platform to identify genes that are down-regulated after expression of mir-493. HCT116 colon cancer cells were transfected with control or mir-493 mimic for 24 hrs, and total RNA extracted from the transfected cells were labeled with Cy3 and used for microarray analyses with Agilent Whole Human Genome Oligo Microarrays.
|
| |
|
| Overall design |
gene expression in HCT116 cells was measured after transfection of control or mir-493. Three independent experiments were performed for each transfection.
|
| |
|
| Contributor(s) |
Okamoto K |
| Citation(s) |
22373578 |
| |
| Submission date |
Aug 30, 2011 |
| Last update date |
Jan 09, 2018 |
| Contact name |
koji okamoto |
| E-mail(s) |
kojokamo@ncc.go.jp
|
| Phone |
81335422511
|
| Organization name |
National Cancer Center Research Institute
|
| Department |
Division of Cancer Differentiation
|
| Street address |
5-1-1 Tsukiji
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1040045 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA145329 |
Less...
More...