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Series GSE32 Query DataSets for GSE32
Status Public on Feb 25, 2002
Title Heatshock otd / OTX2
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary The homeobox genes of the orthodenticle (otd)/Otx family play conserved roles in embryogenesis of the head and brain. Gene replacement experiments show that the Drosophila otd gene and orthologous mammalian Otx genes are functionally equivalent, in that overexpression of either gene in null mutants of Drosophila or mouse can restore defects in cephalic and brain development. This suggests that otd and Otx genes can control a comparable subset of downstream target genes in either organism. Here we use quantitative transcript imaging to analyze this equivalence of Drosophila otd and human Otx gene action at a genomic level.
In this study, transgenic fly strains carrying the otd coding sequence or the human Otx2 coding sequence under control of the heat inducible Hsp70 promoter were used [Leuzinger et al.: Equivalence of the fly orthodenticle gene and the human OTX genes in embryonic brain development of Drosophila. Development 1998; 125:1703-1710.]. Stage 10-17 embryos were given a 25-min heat pulse in order to overexpress the otd or Otx2 genes and allowed to recover for 25 min.
Keywords = Drosophila melanogaster, embryo, brain, development, otd, OTX, heatshock
Keywords: ordered
 
 
Contributor(s) Montalta-He H, Leemans R, Loop T, Strahm M, Certa U, Primig M, Acampora D, Simeone A, Reichert H
Citation(s) 11983056
Submission date Feb 21, 2002
Last update date Dec 05, 2012
Contact name Thomas Loop
E-mail(s) thomas.loop@unibas.ch
Phone +41612671616
URL http://www.unibas.ch/dib/zoologie/research/neuro.html
Organization name University of Basel
Department Institute of Zoology
Lab Reichert Lab
Street address Klingelbergstrasse 50
City Basel
ZIP/Postal code CH-4056
Country Switzerland
 
Platforms (1)
GPL70 Affymetrix roDROMEGAa Drosophila full genome
Samples (16)
GSM1351 hsotd_1
GSM1352 hsotd_2
GSM1353 hsotd_3
Relations
BioProject PRJNA84803

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

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