|
| Status |
Public on Nov 10, 2011 |
| Title |
BMP and Activin treatment of mouse extraembryonic endoderm (XEN) cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
XEN cells are derived from the primitive endoderm of mouse blastocysts. In culture and in chimeras they exhibit properties of parietal endoderm. However, BMP signaling promotes XEN cells to form an epithelium and differentiate into visceral endoderm (VE). Of the several different subtypes of VE described, BMP induces a subtype that is most similar to the VE adjacent to the trophoblast-derived extraembryonic ectoderm.
The experiment was performed to gain insight into genes regulated by BMP and activin in XEN cells, and also to more precisely define the VE subtypes formed in culture.
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| |
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| Overall design |
IM8A1 XEN cells were treated for 6 days with BMP2 (20 ng/ml, R&D Systems), activin A (30 ng/ml, Peprotech), both, or neither in GMEM + 10% fetal bovine serum.
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| |
|
| Contributor(s) |
Sequin C, Rossant J, Kunath T |
| Citation(s) |
22027433 |
| |
| Submission date |
Sep 18, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tilo Kunath |
| E-mail(s) |
tilo.kunath@ed.ac.uk
|
| Phone |
+44 (0) 131 651 9500
|
| Organization name |
University of Edinburgh
|
| Department |
Centre for Regenerative Medicine
|
| Street address |
5 LIttle France Drive
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA147383 |
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