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Series GSE3227 Query DataSets for GSE3227
Status Public on Sep 02, 2005
Title Global gene expression profiling during pituitary thyrotroph, somatotroph, and lactotroph differentiation.
Organism Gallus gallus
Experiment type Expression profiling by array
Summary The anterior pituitary is comprised of five major hormone-secreting cell types which differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the neuroendocrine system were produced and used to identify genes with potential involvement in onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription during chick embryonic development. We identified 352 cDNAs that were differentially expressed (P < 0.05 and the highest mean at least 1.6-fold greater than the lowest mean) on embryonic day 10, 12, 14, or 17, the period of thyrotroph, somatotroph, and lactotroph differentiation in the chicken. Consistent with cellular ontogeny in the chick anterior pituitary gland, thyroid-stimulating hormone beta-subunit mRNA increased steadily between embryonic day 10 and embryonic day 17, growth hormone mRNA increased between embryonic day 12 and embryonic day 17, and PRL mRNA did not increase until embryonic day 17. Expression of 149 cDNAs increased in a manner similar to thyroid-stimulating hormone beta-subunit mRNA and 67 decreased between embryonic day 10 and embryonic day 17, and some of these are likely associated with thyrotroph differentiation. Similarly, expression of 74 and 66 genes changed in a manner over the 4 ages that is consistent with them having a potential role in induction of growth hormone and prolactin mRNA, respectively. Among these candidate genes are numerous transcription factors and signaling molecules that have not previously been implicated in pituitary development.
Keywords: time course during development (4 ages)
 
Overall design Four biological replicates each of chicken pituitary embryonic day 10, embryonic day 12, embryonic day 14, and embryonic day 17 amplified antisense RNA were analyzed using neuroendocrine cDNA microarrays in a reference design. Each experimental sample (embryonic day 10, 12, 14, or 17) was labeled with Cy3 and hybridized with an aliquot of the Cy5 reference pool, which was generated from all sixteen RNA samples.
 
Contributor(s) Ellestad LE, Carre W, Muchow M, Jenkins SA, Wang X, Cogburn LA, Porter TE
Citation(s) 16493019
Submission date Aug 30, 2005
Last update date Sep 10, 2014
Contact name Tom Eric Porter
E-mail(s) teporter@umd.edu
Phone 301-405-2516
Fax 301-405-7980
URL http://www.ansc.umd.edu/faculty/tpresearch.htm
Organization name University of Maryland
Department Animal and Avian Sciences
Street address Building #142
City College Park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platforms (1)
GPL1744 Chicken_Neuroendocrine_System_5K
Samples (16)
GSM72701 Chicken pituitary gland, embryonic day 10, replicate 1 vs. pituitary reference pool (embryonic days 10, 12, 14, 17)
GSM72702 Chicken pituitary gland, embryonic day 10, replicate 2 vs. pituitary reference pool (embryonic days 10, 12, 14, 17)
GSM72703 Chicken pituitary gland, embryonic day 10, replicate 3 vs. pituitary reference pool (embryonic days 10, 12, 14, 17)
Relations
BioProject PRJNA92753

Summary table header descriptions
ID_REF
Probability (P=)
embryonic day 10 Log Ratio [log2(normalized Cy3/raw Cy5)
embryonic day 12 Log Ratio [log2(normalized Cy3/raw Cy5)
embryonic day 114 Log Ratio [log2(normalized Cy3/raw Cy5)
embryonic day 17 Log Ratio [log2(normalized Cy3/raw Cy5)
fold difference (max - min)

Data table
ID_REF Probability (P=) embryonic day 10 embryonic day 12 embryonic day 114 embryonic day 17 fold difference (max - min)
1 0.540562554 0.172556556 0.224978744 -0.156971845 0.08357317 0.381950589
2 0.073935408 0.308707013 -0.100058184 0.033994959 -0.379532793 0.688239806
3 0.00083014 -0.318860146 -0.253519393 0.331063176 0.844249564 1.16310971
4 0.82338311 0.007623035 -0.12676996 0.004850298 -0.123669357 0.134392996
5 0.001939822 0.471022125 0.273887968 0.048420792 -0.166984542 0.638006667
6 0.536348407 0.024255597 0.209041675 0.276589057 0.27248133 0.25233346
7 0.136960973 0.072267393 0.031876784 -0.112873077 -0.068171445 0.18514047
8 0.960937404 -0.088817744 -0.134959653 -0.090564876 -0.069545919 0.065413734
9 0.000502571 -0.964920839 -1.130803397 -0.257669304 0.969493709 2.100297106
10 0.006486835 0.338363757 0.056320556 -0.039827695 0.057279968 0.378191451
11 0.861300471 0.150317205 0.14485242 0.191789196 0.096338171 0.095451025
12 0.131672833 -0.002906314 0.130990501 0.059017963 0.173690536 0.176596851
13 0.569094013 0.021542095 0.116653985 -0.057199604 0.121684912 0.178884516
14 0.183950737 0.081572382 0.22775499 0.173592624 0.006607829 0.221147161
15 0.00359276 0.154004223 0.397699119 0.374204156 -0.264275265 0.661974384
16 0.029000866 0.321875489 -0.075392119 -0.244432476 -0.308785385 0.630660875
17 0.945788938 0.064146192 0.116704148 0.07359929 0.021215674 0.095488474
18 0.318050201 0.079469775 0.292433942 0.107607611 0.337096174 0.257626399
19 0.446146981 0.115245478 0.330927736 0.005517737 0.399295611 0.393777874
20 0.333145695 0.165710728 0.456112091 0.311248031 0.341708942 0.290401363

Total number of rows: 7194

Table truncated, full table size 555 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3227_RAW.tar 456.9 Mb (http)(custom) TAR (of TIFF)

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