|
| Status |
Public on Oct 10, 2011 |
| Title |
Transposon-based construction of strand-specific RNA-seq libraries |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have developed two methods for efficiently consructing RNA-seq libraries using transposition. Each method constructs high quality RNA-seq libraries when compared to standard approaches. One of the methods (Directional Tn-RNA-seq) maintains strand-of-origin information and exhibits strand specificity comparable to current approaches.
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| |
|
| Overall design |
RNA-seq libraries were constructed from ECC-1, a human cell line, and Universal Human Reference RNA using transposon-based and standard RNA-seq library construciton methods.
|
| |
|
| Contributor(s) |
Gertz J, Myers RM |
| Citation(s) |
22128135 |
| |
| Submission date |
Sep 22, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason Gertz |
| E-mail(s) |
jgertz@hudsonalpha.com
|
| Organization name |
HudsonAlpha Institute
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| SRA |
SRP008331 |
| BioProject |
PRJNA147303 |
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