NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE32370 Query DataSets for GSE32370
Status Public on Apr 06, 2012
Title Regulation of monocyte functional heterogeneity by miR-146a
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Monocytes and their lineage descendants serve as a central defense system against infection and injury but if uncontrolled can also promote an excessive pathological inflammatory response. Therefore a current research goal is to understand how the organism controls the number and function of monocytes and how these variables can be tailored in therapy. Considering the evidence that monocytes are heterogeneous and exist in at least two subsets committed to divergent functions, we investigated whether distinct factors regulate the balance between monocyte subset responses in vivo. We identified a microRNA (miRNA), miR-146a, which is differentially regulated both in mouse (Ly-6Chi and Ly-6Clo) and human (CD14hi and CD14lo CD16+) monocyte subsets. The single miRNA was found to significantly control the amplitude of the Ly-6Chi monocyte response during inflammatory challenge whereas it did not affect Ly-6Clo cells. miR-146a–mediated regulation was cell-intrinsic and depended on Relb, a member of the non-canonical NF-κB/Rel family, which is identified here as a novel miR-146a target. These observations provide novel mechanistic insights into the molecular events that regulate monocyte functional heterogeneity and identify therapeutic targets that can influence the quality and quantity of monocyte responses.
 
Overall design 4 samples of splenic Ly-6Chi monocytes, 4 samples of splenic Ly-6Clo monocytes; both isolated from C57BL/6 mice. Each sample was generated by fluorecsence activated cell sorting from the pooled spleens of 10 mice.
 
Contributor(s) Etzrodt M, Cortez-Retamozo V, Newton A, Zhao J, Ng A, Wildgruber M, Wurdinger T, Xavier R, Geissmann F, Meylan E, Nahrendorf M, Swirski FK, Baltimore D, Weissleder R, Pittet MJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 26, 2011
Last update date Apr 07, 2012
Contact name Martin Etzrodt
E-mail(s) ETZRODT.MARTIN@MGH.HARVARD.EDU
Phone (617) 643-0500
Fax (617) 643-6133
URL http://csb.mgh.harvard.edu/pittet
Organization name Massachusetts General Hospital
Department Center for Systems Biology
Lab Pittet Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL8180 Illumina Mouse v1 MicroRNA expression beadchip
Samples (6)
GSM801423 Ly-6Chi monocyte, steady state - sample 1
GSM801424 Ly-6Chi monocyte, steady state - sample 2
GSM801425 Ly-6Chi monocyte, steady state - sample 3
This SubSeries is part of SuperSeries:
GSE32392 Regulation of Monocyte Functional Heterogeneity by miR-146a
Relations
BioProject PRJNA154527

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32370_RAW.tar 30.0 Kb (http)(custom) TAR
GSE32370_non-normalized_data.txt.gz 19.4 Kb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.