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Series GSE32495 Query DataSets for GSE32495
Status Public on Oct 01, 2011
Title Expression profiling of 3D 2D MDCKII epithelial morphogenesis
Organism Canis lupus familiaris
Experiment type Expression profiling by array
Summary Formation of epithelial tissues requires the generation of apical-basal polarity and the co-ordination of this polarity between neighboring cells to form a central lumen. MDCK cell line has proven to be a powerful model to study mammalian polarized epithelia in vitro. MDCK cells plated in extracellular matrix (ECM) form cysts, a spherical structure of polarized cells enclosing a central lumen which resembles epithelial tubular structures.
The morphogenetic process requires drastic changes in cell architecture, which are regulated by change in gene expression. We used microarrays to identify genes up-regulated in lumen formation. The identification of up-regulated genes could lead us to characterize novel pathways needed for this process.
 
Overall design MDCKII cells were plated in two different conditions: Cells cultured in confluence in plastic dishes, forming polarized monolayers (2D); or cells cultured in plastic dishes covered with Matrigel (ECM) forming three dimensional cysts (3D). Comparison of both transcriptomic profiles would lead us to identify up-regulated genes in the 3D condition, which would be good candidates to be key regulators of novel processes involved in lumen morphogenesis.
 
Contributor(s) Rodriguez-Fraticelli AE, Galvez-Santisteban M, Martin-Belmonte F
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Submission date Sep 29, 2011
Last update date Mar 23, 2012
Contact name Manuel Galvez-Santisteban
E-mail(s) mgalvez@cbm.uam.es
Phone 0034654862430
Organization name CSIC-CBM Severo Ochoa
Department Cell Biology and Immunology
Lab 325
Street address Nicolas Cabrera 1
City Madrid
State/province Madrid
ZIP/Postal code 28049
Country Spain
 
Platforms (1)
GPL3738 [Canine_2] Affymetrix Canine Genome 2.0 Array
Samples (6)
GSM804372 MDCKII, 2D, biological rep1
GSM804373 MDCKII, 2D, biological rep2
GSM804374 MDCKII, 2D, biological rep3
Relations
BioProject PRJNA147767

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32495_RAW.tar 22.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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