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Series GSE32663 Query DataSets for GSE32663
Status Public on Oct 06, 2011
Title Identification of 67 histone marks and histone lysine crotonylation as a new type of histone modification
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the identification of 67 previously undescribed histone modifications, increasing the current number of known histone marks by about 70%. We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslational modification. The unique structure and genomic localization of histone Kcr suggest that it is mechanistically and functionally different from histone lysine acetylation (Kac). Specifically, in both human somatic and mouse male germ cell genomes, histone Kcr marks either active promoters or potential enhancers. In male germinal cells immediately following meiosis, Kcr is enriched on sex chromosomes and specifically marks testis-specific genes, including a significant proportion of X-linked genes that escape sex chromosome inactivation in haploid cells. These results therefore dramatically extend the repertoire of histone PTM sites and designate Kcr as a specific mark of active sex chromosome-linked genes in postmeiotic male germ cells.
Overall design 2 histone marks (pan-lysine acetylation and pan-lysine crotonylation) were studied in 1 human cell type and 2 mouse cell types using ChIP-Seq. Input was sequenced for each cell type as a control. Pan-anti_Kac and pan-anti_Kcr antibodies were custom developed with PTM BioLab, Co., Ltd (Chicago, IL).
Contributor(s) Fulai J, Khochbin S, Ren B, Zhao Y
Citation(s) 21925322
Submission date Oct 06, 2011
Last update date May 15, 2019
Contact name Fulai Jin
Phone 2163681811
Organization name Case Western Reserve University
Lab Fulai Jin
Street address 10900 Euclid Avenue
City Cleveland
State/province OH - OHIO
ZIP/Postal code 44106
Country USA
Platforms (3)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM810670 IMR90_input
GSM810671 IMR90_pan-lysine-acetylation
GSM810672 IMR90_Kcro
SRA SRP008731
BioProject PRJNA147047

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Supplementary file Size Download File type/resource
GSE32663_RAW.tar 2.1 Gb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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