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Status |
Public on Oct 14, 2011 |
Title |
Catabolite repression of phosphoenolpyruvate carboxykinase by a zinc finger protein under biotin- and pyruvate carboxylase-deficient conditions in Pichia pastoris |
Organism |
Komagataella pastoris |
Experiment type |
Expression profiling by array
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Summary |
We have identified a methanol- and biotin-starvation-inducible zinc finger protein named ROP [repressor of phosphoenolpyruvate carboxykinase (PEPCK)] in the methylotrophic yeast Pichia pastoris. When P.pastoris strain GS115 (wild-type, WT) is cultured in biotin-deficient, glucose ammonium (Bio-) medium, growth is suppressed due to the inhibition of anaplerotic synthesis of oxaloacetate, catalysed by the biotin-dependent enzyme pyruvate carboxylase (PC). Deletion of ROP results in a strain (∆ROP) that can grow under biotin-deficient conditions due to derepression of a biotin- and PC-independent pathway of anaplerotic synthesis of oxaloacetate. Northern analysis as well as microarray expression profiling of RNA isolated from WT and ∆ROP strains cultured in Bio(-) medium indicate that expression of the phosphoenolpyruvate carboxykinase gene (PEPCK) is induced in ∆ROP during biotin- or PC-deficiency even under glucose-abundant conditions. There is an excellent correlation between PEPCK expression and growth of ∆ROP in Bio(-) medium, suggesting that ROP-mediated regulation of PEPCK may have a crucial role in the biotin- and PC-independent growth of the ∆ROP strain. To our knowledge, ROP is the first example, of a zinc finger transcription factor involved in the catabolite repression of PEPCK in yeast cells cultured under biotin- or PC-deficient and glucose-abundant conditions.
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Overall design |
Agilent one-color experiment,Organism: Pichia pastoris ,Custom Pichia pastoris Gene Expression 8x15k array designed by Genotypic Technology Pvt. Ltd. (Agilent -AMADID: 25088 ) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
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Contributor(s) |
Kumar NV, Rangarajan PN |
Citation(s) |
21948049 |
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Submission date |
Oct 13, 2011 |
Last update date |
Mar 23, 2012 |
Contact name |
Genotypic technology |
E-mail(s) |
sudha.rao@genotypic.co.in
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Organization name |
Genotypic Technology
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Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
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City |
Bangalore |
State/province |
Karnataka |
ZIP/Postal code |
560094 |
Country |
India |
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Platforms (1) |
GPL14714 |
Agilent-025088 Pichia pastoris Gene Expression 8x15k array |
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Samples (4)
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Relations |
BioProject |
PRJNA146463 |