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| Status |
Public on Oct 22, 2011 |
| Title |
Human bone marrow precursor cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The aim of this study was to characterize in vivo miRNA expression in normal myeloid development of the neutrophil granulocyte (granulopoiesis) to gain insight into miRNA control of these processes. Cell populations highly enriched in precursors from successive stages of granulopoiesis and mature neutrophils were isolated from bone marrow (BM) and peripheral blood (PB) samples, respectively, from 4 healthy human donors. Total RNA was extracted from each cell population and subjected to miRNA gene expression profiling using miRCURYTM LNA microRNA Arrays
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| Overall design |
Total RNA from cell populations highly enriched in precursors from three succesive stages of neutrophil granulopoiesis and mature neutrophils isolated from bone marrow and peripheral blood, respectively, from four healthy donors (SKP, AJ, AO, JO). The three cell populations from bone marrow where extracted and named B3, B2, and B1, corresponding to immature (myeloblasts [MBs] and promyelocytes [PMs]); intermediate mature (myelocytes [MCs] and metamyelocytes [MMs]); and mature neutrophil cells (band cells [BCs] and segmented neutrophil cells [SCs]), respectively, as described in Bjerregaard MD, Jurlander J, Klausen P, Borregaard N, Cowland JB: The in vivo profile of transcription factors during neutrophil differentiation in human bone marrow. Blood 2003, 101: 4322-4332. In total, 16 samples were compared (4 cell maturation stages from 4 donors)
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| Contributor(s) |
Pedersen CC, Jarmer H, Hother C, Grønbæk K, Borregaard N, Cowland JB |
| Citation(s) |
22028478 |
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| Submission date |
Oct 21, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Corinna Cavan Pedersen |
| E-mail(s) |
corinnacp@yahoo.com
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| Organization name |
Rigshospitalet
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| Street address |
Juliane Mariesvej 20, 9322
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| City |
Copenhagen |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
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| Platforms (1) |
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| Samples (16)
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| GSM820709 |
mature_bone_marrow_BCs_and_SCs_SKP.B1 |
| GSM820710 |
immature_bone_marrow_MBs_and_PMs_AO.B3 |
| GSM820711 |
Peripheral_blood_PMNs_AJ.PMN |
| GSM820712 |
intermediate_mature_bone_marrowMCs_and_MMs_JO.B2 |
| GSM820713 |
immature_bone_marrow_MBs_and_PMs_AJ.B3 |
| GSM820714 |
mature_bone_marrow_BCs_and_SCs_JO.B1 |
| GSM820715 |
intermediate_mature_bone_marrowMCs_and_MMs_SKP.B2 |
| GSM820716 |
Peripheral_blood_PMNs_AO.PMN |
| GSM820717 |
intermediate_mature_bone_marrowMCs_and_MMs_AO.B2 |
| GSM820718 |
Peripheral_blood_PMNs_SKP.PMN |
| GSM820719 |
immature_bone_marrow_MBs_and_PMs_JO.B3 |
| GSM820720 |
mature_bone_marrow_BCs_and_SCs_AJ.B1 |
| GSM820721 |
Peripheral_blood_PMNs_JO.PMN |
| GSM820722 |
intermediate_mature_bone_marrowMCs_and_MMs_AJ.B2 |
| GSM820723 |
mature_bone_marrow_BCs_and_SCs_AO.B1 |
| GSM820724 |
immature_bone_marrow_MBs_and_PMs_SKP.B3 |
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| Relations |
| BioProject |
PRJNA149387 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33140_US22502610_13638666_S01_Cropped_001.gpr.gz |
532.8 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13638669_S01_Cropped_001.gpr.gz |
542.9 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639002_S01_Cropped_001.gpr.gz |
509.1 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639007_S01_Cropped_001.gpr.gz |
528.9 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639009_S01_Cropped_001.gpr.gz |
519.0 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639010_S01_Cropped_001.gpr.gz |
520.7 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639011_S01_Cropped_001.gpr.gz |
557.4 Kb |
(ftp)(http) |
GPR |
| GSE33140_US22502610_13639013_S01_Cropped_001.gpr.gz |
551.5 Kb |
(ftp)(http) |
GPR |
| Processed data included within Sample table |
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