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Series GSE33140 Query DataSets for GSE33140
Status Public on Oct 22, 2011
Title Human bone marrow precursor cells
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The aim of this study was to characterize in vivo miRNA expression in normal myeloid development of the neutrophil granulocyte (granulopoiesis) to gain insight into miRNA control of these processes. Cell populations highly enriched in precursors from successive stages of granulopoiesis and mature neutrophils were isolated from bone marrow (BM) and peripheral blood (PB) samples, respectively, from 4 healthy human donors. Total RNA was extracted from each cell population and subjected to miRNA gene expression profiling using miRCURYTM LNA microRNA Arrays
Overall design Total RNA from cell populations highly enriched in precursors from three succesive stages of neutrophil granulopoiesis and mature neutrophils isolated from bone marrow and peripheral blood, respectively, from four healthy donors (SKP, AJ, AO, JO). The three cell populations from bone marrow where extracted and named B3, B2, and B1, corresponding to immature (myeloblasts [MBs] and promyelocytes [PMs]); intermediate mature (myelocytes [MCs] and metamyelocytes [MMs]); and mature neutrophil cells (band cells [BCs] and segmented neutrophil cells [SCs]), respectively, as described in Bjerregaard MD, Jurlander J, Klausen P, Borregaard N, Cowland JB: The in vivo profile of transcription factors during neutrophil differentiation in human bone marrow. Blood 2003, 101: 4322-4332. In total, 16 samples were compared (4 cell maturation stages from 4 donors)
Contributor(s) Pedersen CC, Jarmer H, Hother C, Grønbæk K, Borregaard N, Cowland JB
Citation(s) 22028478
Submission date Oct 21, 2011
Last update date Mar 23, 2012
Contact name Corinna Cavan Pedersen
Organization name Rigshospitalet
Street address Juliane Mariesvej 20, 9322
City Copenhagen
ZIP/Postal code 2100
Country Denmark
Platforms (1)
GPL13273 EXIQON miRCURY™ V8.1 LNA array
Samples (16)
GSM820709 mature_bone_marrow_BCs_and_SCs_SKP.B1
GSM820710 immature_bone_marrow_MBs_and_PMs_AO.B3
GSM820711 Peripheral_blood_PMNs_AJ.PMN
BioProject PRJNA149387

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33140_US22502610_13638666_S01_Cropped_001.gpr.gz 532.8 Kb (ftp)(http) GPR
GSE33140_US22502610_13638669_S01_Cropped_001.gpr.gz 542.9 Kb (ftp)(http) GPR
GSE33140_US22502610_13639002_S01_Cropped_001.gpr.gz 509.1 Kb (ftp)(http) GPR
GSE33140_US22502610_13639007_S01_Cropped_001.gpr.gz 528.9 Kb (ftp)(http) GPR
GSE33140_US22502610_13639009_S01_Cropped_001.gpr.gz 519.0 Kb (ftp)(http) GPR
GSE33140_US22502610_13639010_S01_Cropped_001.gpr.gz 520.7 Kb (ftp)(http) GPR
GSE33140_US22502610_13639011_S01_Cropped_001.gpr.gz 557.4 Kb (ftp)(http) GPR
GSE33140_US22502610_13639013_S01_Cropped_001.gpr.gz 551.5 Kb (ftp)(http) GPR
Processed data included within Sample table

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