|
| Status |
Public on Jan 11, 2012 |
| Title |
The amniotic fluid transcriptome: a source of novel information about human fetal development |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Amniotic fluid (AF) is a complex biological material that provides a unique window into the developing human. Residual AF supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the AF core transcriptome by identifying the transcripts ubiquitously present in the AF supernatant of euploid midtrimester fetuses.
We detected 476 well-annotated genes present in all twelve AF samples. Functional analysis identified 6 physiological systems represented in the AF core transcriptome, including musculoskeletal and nervous system development and function and embryonic/ organismal development. mTOR signaling was identified as a key canonical pathway. Twenty-three highly organ-specific transcripts were identified; six of these are known to be highly expressed by fetal brain. This study demonstrates that AF cell-free fetal RNA can provide biological information about multiple fetal organ systems.
|
| |
|
| Overall design |
This was an in silico investigation of gene expression in 12 euploid midtrimester AF samples acquired primarily for advanced maternal age. We focused on the transcripts called “present” in all of the samples. Functional analyses were performed using Ingenuity software. Fetal-organ specificity was examined for each transcript using the GNF Gene Expression Atlas. For fetal organs not represented in the atlas, manual literature searching and Ingenuity analysis were used.
|
| |
|
| Contributor(s) |
Hui L, Slonim DK, Wick HC, Johnson KL, Bianchi DW |
| Citation(s) |
22183218 |
| Submission date |
Oct 24, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Donna K Slonim |
| E-mail(s) |
Donna.Slonim@tufts.edu
|
| Organization name |
Tufts University
|
| Department |
Computer Science
|
| Street address |
161 College Ave.
|
| City |
Medford |
| State/province |
MA |
| ZIP/Postal code |
01845 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
| GSM821262 |
second_trimester_euploid_AF_normalAFstudy_female1 |
| GSM821263 |
second_trimester_euploid_AF_normalAFstudy_female2 |
| GSM821264 |
second_trimester_euploid_AF_normalAFstudy_female3 |
| GSM821265 |
second_trimester_euploid_AF_normalAFstudy_female4 |
| GSM821266 |
second_trimester_euploid_AF_normalAFstudy_female5 |
| GSM821267 |
second_trimester_euploid_AF_normalAFstudy_female6 |
| GSM821268 |
second_trimester_euploid_AF_normalAFstudy_male1 |
| GSM821269 |
second_trimester_euploid_AF_normalAFstudy_male2 |
| GSM821270 |
second_trimester_euploid_AF_normalAFstudy_male3 |
| GSM821271 |
second_trimester_euploid_AF_normalAFstudy_male4 |
| GSM821272 |
second_trimester_euploid_AF_normalAFstudy_male5 |
| GSM821273 |
second_trimester_euploid_AF_normalAFstudy_male6 |
|
| Relations |
| BioProject |
PRJNA149231 |
Less...
More...