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| Status |
Public on Sep 17, 2005 |
| Title |
Differential protein expression by Porphyromonas gingivalis in response to secreted epithelial cell components |
| Organism |
Porphyromonas gingivalis |
| Experiment type |
Other
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| Summary |
The human oral pathogen Porphyromonas gingivalis colonizes the gingival crevice and invades gingival epithelial cells. Multidimensional capillary high-performance liquid chromatography coupled with tandem mass spectrometry and two-dimensional gel electrophoresis were used to analyze the proteome of P. gingivalis as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were identified in four independent analyses; 479 of these proteins showed evidence of differential expression after exposure of P. gingivalis to either conditioned epithelial cell growth medium or control conditions: i.e., they were only detected under one set of conditions. Moreover, 276 genes annotated as hypothetical were found to encode expressed proteins. Among the proteins up-regulated in the presence of epithelial cell components were a homolog of the internalin proteins of Listeria monocytogenes and subunits of the ATP-dependent Clp protease complex. Insertional inactivation of clpP, encoding the Clp proteolytic subunit, resulted in approximately a 50% reduction in invasion of P. gingivalis. These results suggest that adaptation to an epithelial cell environment induces a major shift in the expressed proteome of the organism. Furthermore, ClpP, that is up-regulated in this environment, is required for optimal invasive activity of P. gingivalis.
Keywords: proteome analysis of P. gingivalis
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| Overall design |
P. gingivalis exposed to control keratinocyte growth medium or conditioned keratinocyte growth medium
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| Contributor(s) |
Zhang Y, Wang T, Chen W, Yilmaz O, Park Y, Jung I, Hackett M, Lamont RJ |
| Citation(s) |
15619293 |
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| Submission date |
Sep 16, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Murray Hackett |
| E-mail(s) |
MHACKETT@U.WASHINGTON.EDU
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| Phone |
206-616-8071
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| Organization name |
University of Washington
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| Department |
Chemical Engineering
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| Lab |
Hackett
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| Street address |
HSB H330 1959 NE Pacific Street
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL2841 |
P. gingivalis cKGM/KGM proteomics study |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA93359 |
| Supplementary data files not provided |
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