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Series GSE3322 Query DataSets for GSE3322
Status Public on Sep 17, 2005
Title Differential protein expression by Porphyromonas gingivalis in response to secreted epithelial cell components
Organism Porphyromonas gingivalis
Experiment type Other
Summary The human oral pathogen Porphyromonas gingivalis colonizes the gingival crevice and invades gingival epithelial cells. Multidimensional capillary high-performance liquid chromatography coupled with tandem mass spectrometry and two-dimensional gel electrophoresis were used to analyze the proteome of P. gingivalis as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were identified in four independent analyses; 479 of these proteins showed evidence of differential expression after exposure of P. gingivalis to either conditioned epithelial cell growth medium or control conditions: i.e., they were only detected under one set of conditions. Moreover, 276 genes annotated as hypothetical were found to encode expressed proteins. Among the proteins up-regulated in the presence of epithelial cell components were a homolog of the internalin proteins of Listeria monocytogenes and subunits of the ATP-dependent Clp protease complex. Insertional inactivation of clpP, encoding the Clp proteolytic subunit, resulted in approximately a 50% reduction in invasion of P. gingivalis. These results suggest that adaptation to an epithelial cell environment induces a major shift in the expressed proteome of the organism. Furthermore, ClpP, that is up-regulated in this environment, is required for optimal invasive activity of P. gingivalis.
Keywords: proteome analysis of P. gingivalis
Overall design P. gingivalis exposed to control keratinocyte growth medium or conditioned keratinocyte growth medium
Contributor(s) Zhang Y, Wang T, Chen W, Yilmaz O, Park Y, Jung I, Hackett M, Lamont RJ
Citation(s) 15619293
Submission date Sep 16, 2005
Last update date Mar 16, 2012
Contact name Murray Hackett
Phone 206-616-8071
Organization name University of Washington
Department Chemical Engineering
Lab Hackett
Street address HSB H330 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platforms (1)
GPL2841 P. gingivalis cKGM/KGM proteomics study
Samples (1)
GSM74211 PG_cKGM/KGM_001
BioProject PRJNA93359

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