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| Status |
Public on Oct 24, 2016 |
| Title |
Gene expression in the tail tissue of tadpoles [Rana pirica] by larvae salamander or dragon fly |
| Platform organism |
Xenopus laevis |
| Sample organism |
Rana pirica |
| Experiment type |
Expression profiling by array
|
| Summary |
Tadpoles of the anuran species Rana pirica can undergo predator-specific morphological responses. Exposure to a predation threat by larvae of the salamander Hynobius retardatus results in formation of a bulgy body (bulgy morph) with a higher tail. Whereas, dragon fly also induced higher tail tadpole. The tadpoles revert to a normal phenotype upon removal of the larval salamander or dragon fly threat.
The objective of the present study was to use Affymetrix Xenopus Genechip to profile gene expression in the tail tissue by different predation threat.
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| Overall design |
Tadpoles of Rana pirica treated with larvae salamander for 8days (S1, S2, S3) or dragon fly for 8days (Y1,Y2, Y3) were analyzed with triplicate. Removal experiments were also treated with predators for 4days and then removed predators from tadpoles (-S1,-S2, -S3) or (-Y1,-Y2,-Y3). Controls were cultured for 8days without predators (C2, C3). Tails from tadpoles after 8days of each treatment were dissected for RNA extraction and gene expression analysis using Affymetrix Xenopus Genechip arrays.
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| Contributor(s) |
Mori T |
| Citation(s) |
25886855 |
| |
| Submission date |
Oct 26, 2011 |
| Last update date |
Oct 11, 2019 |
| Contact name |
Tsukasa Mori |
| E-mail(s) |
mori.tsukasa@nihon-u.ac.jp
|
| Phone |
01-(81)-466-84-3682
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| Fax |
01-(81)-466-84-3682
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| URL |
http://01-(81)-466-84-3682
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| Organization name |
Nihon University
|
| Department |
Bioresource Sciences
|
| Lab |
Marine Molecular Biochemistry
|
| Street address |
Kameino 1866
|
| City |
Fujisawa |
| State/province |
Kanagawa |
| ZIP/Postal code |
252−0880 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
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| Samples (14)
|
| GSM822840 |
Salamander: biological rep2 |
| GSM822841 |
Salamander: biological rep3 |
| GSM822842 |
Dragon fly: biological rep1 |
| GSM822843 |
Dragon fly: biological rep2 |
| GSM822844 |
Dragon fly: biological rep3 |
| GSM822845 |
Removal salamander after 4 days: biological rep1 |
| GSM822846 |
Removal salamander after 4 days: biological rep2 |
| GSM822847 |
Removal salamander after 4 days: biological rep3 |
| GSM822848 |
Removal dragon fly after 4 days: biological rep1 |
| GSM822849 |
Removal dragon fly after 4 days: biological rep2 |
| GSM822850 |
Removal dragon fly after 4 days: biological rep3 |
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| Relations |
| BioProject |
PRJNA149073 |
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