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| Status |
Public on Jan 31, 2012 |
| Title |
Pyrococcus furiosus crRNAs associated with the Cas RAMP module complex |
| Organism |
Pyrococcus furiosus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of CRISPR RNAs from both total Pyrococcus furiosus RNA and those that co-purify with the Cmr complex, which has been predicted to be involved in RNA-mediated targeting of foreign RNAs in prokaryotes. Small RNAs (20 - 70 nts) were isolated either from total Pyrococcus furiosus RNA or RNAs isolated from immunopurified Cmr complexes. The RNAs were cloned and sequenced using the Illumina platform. Results provide insight into the anatomy of Cmr2-associated CRISPR RNAs.
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| Overall design |
2 Samples: total small RNAs vs Cmr2-associated small RNAs
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| Contributor(s) |
Graveley BR, Hale CR, Majumdar S, Elmore J, Pfister N, Compton M, Olson S, Resch AM, Glover III CV, Terns R, Terns M |
| Citation(s) |
22227116, 25904135 |
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| Submission date |
Oct 26, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Brenton R Graveley |
| E-mail(s) |
graveley@uchc.edu
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| Phone |
860-679-2090
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| Organization name |
University of Connecticut Health Center
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| Department |
Genetics and Genome Sciences
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| Street address |
400 Farmington Avenue
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| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030-3301 |
| Country |
USA |
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| Platforms (1) |
| GPL14794 |
Illumina Genome Analyzer IIx (Pyrococcus furiosus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP009098 |
| BioProject |
PRJNA149041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33256_RAW.tar |
37.0 Mb |
(http)(custom) |
TAR (of BEDGRAPH, SAM) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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