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| Status |
Public on Nov 01, 2011 |
| Title |
Transcriptomic and functional analysis of the Anopheles gambiae salivary gland in relation to blood feeding |
| Organism |
Anopheles gambiae |
| Experiment type |
Expression profiling by array
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| Summary |
Background: The Anopheles gambiae salivary glands play a major role in malaria transmission and express a variety of bioactive components that facilitate blood-feeding by preventing platelet aggregation, blood clotting, vasodilatation, and inflammatory and other reactions at the probing site on the vertebrate host.
Results: We have performed a global transcriptome analysis of the A. gambiae salivary gland response to blood-feeding, to identify candidate genes that are involved in hematophagy. A total of 4,978 genes were found to be transcribed in this tissue. A comparison of salivary gland transcriptomes prior to and after blood-feeding identified 52 and 41 transcripts that were significantly up-regulated and down-regulated, respectively. Ten genes were further selected to assess their role in the blood-feeding process using RNAi-mediated gene silencing methodology. Depletion of the salivary gland genes encoding D7L2, anophelin, peroxidase, the SG2 precursor, and a 5'nucleotidase gene significantly increased probing time of A. gambiae mosquitoes and thereby their capacity to blood-feed.
Conclusions: The salivary gland transcriptome comprises approximately 38% of the total mosquito transcriptome and a small proportion of it is dynamically changing already at two hours in response to blood feeding. A better understanding of the salivary gland transcriptome and its function can contribute to the development of pathogen transmission control strategies and the identification of medically relevant bioactive compounds.
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| Overall design |
Salivary glands from blood-fed vs. unfed A. gambiae. 3 replicates.
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| Contributor(s) |
Dimopoulos G |
| Citation(s) |
20946652 |
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| Submission date |
Nov 01, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
George Dimopoulos |
| E-mail(s) |
gdimopo1@jhu.edu
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| Phone |
443 28 70128
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| Organization name |
Johns Hopkins School of Public Health
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| Department |
Molecular Microbiology and Immunology
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| Street address |
615 N. Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
| GSM573605 |
Blood-fed salivary gland vs. unfed salivary gland, rep1 |
| GSM573606 |
Blood-fed salivary gland vs. unfed salivary gland, rep2 |
| GSM573607 |
Blood-fed salivary gland vs. unfed salivary gland, rep3 |
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| Relations |
| BioProject |
PRJNA149113 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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