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| Status |
Public on Dec 16, 2012 |
| Title |
Genome-wide analysis of the BR response in msg2 and axr2 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Although a wide range of interactions between BRs and auxin have been recognized, knowledge about the direct molecular mechanism of interaction between them in specific physiological processes is very limited. In this study we found that auxin resisitent mutant msg2/iaa19 and arf7 were also resisitant to the BR effect on morphogenensis of dark-grown Arabidosis seedlings. Moreover, BR signaling transcription factor BZR1 can directly bind to promoter regions of IAA19 and ARF7.
Microarray analysis revealed that a number of gene transcripts showed reduced BR response in msg2 and the control mutant axr2, suggesting the crucial role of IAA19 in mediating BR effects. Taken together, our results suggested that BRs regulate morphogenesis of dark-grown seedling by employing auxin signaling components IAA19 and ARF7.
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| Overall design |
Four-day-old seedlings of WT, msg2 and axr2 grown on MS medium in the absence or presence of 24-eBL (100 nM) were used. Total RNA extraction and ATH1 microarray(Affymetrix) experiments were performend according to manufacturer’s protocols. Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0. All experiments including two biological replicates and the Pearson correlation coefficient was analyzed to check the replicate quality.
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| Contributor(s) |
Zhou X, Xue H |
| Citation(s) |
23125315 |
| Submission date |
Nov 02, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
zhou xiao yi |
| E-mail(s) |
zhouxy@sibs.ac.cn
|
| Phone |
021-54924062
|
| Organization name |
Shanghai Institutes for Biological Science
|
| Street address |
300 fenglin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA148941 |
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