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Status |
Public on Dec 12, 2011 |
Title |
miR-124 acts through coREST to control the onset of Sema3A sensitivity in navigating retinal growth cones |
Organism |
Xenopus laevis |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
During axon pathfinding, growth cones commonly exhibit changes in sensitivity to guidance cues that follow a strict timetable, even in the absence of pathway feedback, implicating cell-intrinsic regulation. Cellular timer mechanisms, however, are poorly understood. Here we have investigated microRNAs in the timing control of Sema3A sensitivity in retinal ganglion cell (RGC) growth cones. A developmental profiling screen identified miR-124 as a candidate timer. Loss of miR-124 delayed the onset of Sema3A sensitivity and concomitant Neuropilin-1 (NRP-1) receptor expression, and caused cell autonomous pathfinding errors. CoREST, a cofactor of a NRP1 repressor, was identified as a novel target and mediator of miR-124 for this highly specific temporal aspect of RGC growth cone responsiveness. Our findings indicate that miR-124 plays an important role in regulating the intrinsic temporal changes in RGC growth cone sensitivity and suggest that microRNAs may play a broad role as linear timers in vertebrate neuronal development.
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Overall design |
Two independent experiments were performed. One with a single sample for each of 3 stages, and the second with 2 biological replicates of each stage.
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Contributor(s) |
Baudet M, Zivraj KH, Abreu-Goodger C, Muldal A, Armisen J, Blenkiron C, Goldstein LD, Miska EA, Holt CE |
Citation(s) |
22138647 |
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Submission date |
Nov 03, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Cei Abreu-Goodger |
E-mail(s) |
cei.abreu@cinvestav.mx
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Organization name |
Langebio - Cinvestav
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Street address |
Km. 9.6 Libramiento Nte Carr Leon-Irapuato
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City |
Irapuato |
State/province |
Guanajuato |
ZIP/Postal code |
36824 |
Country |
Mexico |
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Platforms (1) |
GPL14834 |
Illumina Genome Analyzer IIx (Xenopus laevis) |
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Samples (9)
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Relations |
SRA |
SRP009183 |
BioProject |
PRJNA148701 |
Supplementary file |
Size |
Download |
File type/resource |
GSE33444_RAW.tar |
74.6 Mb |
(http)(custom) |
TAR (of FA) |
GSE33444_miRNA_mapped_counts_xenTro2.txt.gz |
4.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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