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Series GSE33444 Query DataSets for GSE33444
Status Public on Dec 12, 2011
Title miR-124 acts through coREST to control the onset of Sema3A sensitivity in navigating retinal growth cones
Organism Xenopus laevis
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary During axon pathfinding, growth cones commonly exhibit changes in sensitivity to guidance cues that follow a strict timetable, even in the absence of pathway feedback, implicating cell-intrinsic regulation. Cellular timer mechanisms, however, are poorly understood. Here we have investigated microRNAs in the timing control of Sema3A sensitivity in retinal ganglion cell (RGC) growth cones. A developmental profiling screen identified miR-124 as a candidate timer. Loss of miR-124 delayed the onset of Sema3A sensitivity and concomitant Neuropilin-1 (NRP-1) receptor expression, and caused cell autonomous pathfinding errors. CoREST, a cofactor of a NRP1 repressor, was identified as a novel target and mediator of miR-124 for this highly specific temporal aspect of RGC growth cone responsiveness. Our findings indicate that miR-124 plays an important role in regulating the intrinsic temporal changes in RGC growth cone sensitivity and suggest that microRNAs may play a broad role as linear timers in vertebrate neuronal development.
 
Overall design Two independent experiments were performed. One with a single sample for each of 3 stages, and the second with 2 biological replicates of each stage.
 
Contributor(s) Baudet M, Zivraj KH, Abreu-Goodger C, Muldal A, Armisen J, Blenkiron C, Goldstein LD, Miska EA, Holt CE
Citation(s) 22138647
Submission date Nov 03, 2011
Last update date May 15, 2019
Contact name Cei Abreu-Goodger
E-mail(s) cei.abreu@cinvestav.mx
Organization name Langebio - Cinvestav
Street address Km. 9.6 Libramiento Nte Carr Leon-Irapuato
City Irapuato
State/province Guanajuato
ZIP/Postal code 36824
Country Mexico
 
Platforms (1)
GPL14834 Illumina Genome Analyzer IIx (Xenopus laevis)
Samples (9)
GSM827025 Stage24_0
GSM827026 Stage24_1
GSM827027 Stage24_2
Relations
SRA SRP009183
BioProject PRJNA148701

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33444_RAW.tar 74.6 Mb (http)(custom) TAR (of FA)
GSE33444_miRNA_mapped_counts_xenTro2.txt.gz 4.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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