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| Status |
Public on Sep 24, 2013 |
| Title |
Expression data from volatile treated and insect damaged Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Plant volatiles can mediate plant-plant communication in the sense that plants attacked by herbivores can signal their unattacked neighbors of danger by emitting HIPVs. We call this the priming effect. Since the plant defense response is a systematic process involving numerous pathways and genes,to characterize the priming process, a time course study using a genome-wide microarray may provide more accurate information about the priming process. Furthermore, to what extent do the priming process and direct defense share similar gene expression profiles or pathways are also not clear.
We used microarray to detect the priming effect of plant volatiles to healthy Arabidopsis thaliana, and the effect of direct leafminer feeding to Arabidopsis thalianas.
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| Overall design |
A system using Lima bean plants, from which HIPVs can be effectively induced by leafminer feeding, as emitters and Arabidopsis thaliana as receivers is used to track the priming process between neighbor plants. The Arabisopsis thaliana seedlings were treated by volatiles from leafminer fed lima bean for 24h or 48h for RNA extraction and hybridization on Affymetrix microarrays. The Arabisopsis thaliana seedlings fed by leafminer directly were also collected The for RNA extraction and hybridization on Affymetrix micorarrays. We want to explore the response of Arabidopsis thaliana to priming volatiles during a 24h-48h time course. We also want to compare the effect of priming and direct leafminer feeding.
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| Contributor(s) |
Kang L, Zhang S, We J |
| Citation(s) |
22558246 |
| Submission date |
Nov 07, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Sufang Zhang |
| E-mail(s) |
wavingsnow@ustc.edu
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| Phone |
86-10-62888896
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| Organization name |
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry
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| Street address |
No.2 Dong Xiao Fu, Wan Shou Shan, Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (16)
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| GSM828851 |
A.thaliana seedlings treated by volatiles from healthy Lima bean plants for 24h rep1 |
| GSM828852 |
A.thaliana seedlings treated by volatiles from healthy Lima bean plants for 24h rep2 |
| GSM828853 |
A.thaliana seedlings treated by volatiles from healthy Lima bean plants for 48h rep1 |
| GSM828854 |
A.thaliana seedlings treated by volatiles from healthy Lima bean plants for 48h rep2 |
| GSM828855 |
A.thaliana seedlings treated by volatiles from healthy Lima bean plants for 48h rep3 |
| GSM828856 |
A.thaliana seedlings treated by volatiles from leafminer damaged Lima bean plants for 24h rep1 |
| GSM828857 |
A.thaliana seedlings treated by volatiles from leafminer damaged Lima bean plants for 24h rep2 |
| GSM828858 |
A.thaliana seedlings treated by volatiles from leafminer damaged Lima bean plants for 48h rep1 |
| GSM828859 |
A.thaliana seedlings treated by volatiles from leafminer damaged Lima bean plants for 48h rep2 |
| GSM828860 |
A.thaliana seedlings treated by volatiles from leafminer damaged Lima bean plants for 48h rep3 |
| GSM828861 |
Healthy A.thaliana seedlings rep1 |
| GSM828862 |
Healthy A.thaliana seedlings rep2 |
| GSM828863 |
Healthy A.thaliana seedlings rep3 |
| GSM828864 |
Leafminer damaged A.thaliana seedlings rep1 |
| GSM828865 |
Leafminer damaged A.thaliana seedlings rep2 |
| GSM828866 |
Leafminer damaged A.thaliana seedlings rep3 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA148837 |
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