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Series GSE33548 Query DataSets for GSE33548
Status Public on Apr 03, 2012
Title SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii
Organism Chlamydomonas reinhardtii
Experiment type Expression profiling by high throughput sequencing
Summary To identify components involved in the signal transduction and activation of the singlet oxygen-mediated response, a mutant selection was performed. This led to the isolation of the singlet oxygen resistant 1 (sor1) mutant, which is more tolerant to singlet oxygen-producing chemicals and shows a constitutively higher expression of GPXH and GSTS1. Map-based cloning revealed that the SOR1 gene encodes a novel bZIP transcription factor, which seems to control its own expression as well as that of a large number of oxidative stress response and detoxification genes. In the promoter region of many of these genes a highly conserved 8-bp palindromic sequence element was found to be enriched. This element was shown to be essential for GSTS1 overexpression in sor1 and for induction by increased levels of lipophilic reactive electrophile species (RES) suggesting that it functions as an electrophile response element (ERE). RES can be formed after singlet oxygen-induced lipid peroxidation, indicating that a RES-stimulated and SOR1-mediated response of detoxification genes is part of the singlet oxygen-induced acclimation process in C. reinhardtii.
Overall design The sor1 mutant isolated in a screen for singlet oxygen resistant mutants and the corresponding wild-type strain 4A+ were grown in a 12 h light dark cycle for several days before total RNA was extracted 6 h after the light came on.
Contributor(s) Fischer BB, Ledford HK, Wakao S, Huang S, Casero D, Pellegrini M, Merchant SS, Koller A, Eggen RI, Niyogi KK
Citation(s) 22529359
Submission date Nov 08, 2011
Last update date May 15, 2019
Contact name Beat B Fischer
Phone +41 44 8235492
Organization name Eawag
Department Department of Environmental Toxicology
Street address Ueberlandstr. 133
City Dübendorf
ZIP/Postal code 8600
Country Switzerland
Platforms (2)
GPL9100 Illumina Genome Analyzer II (Chlamydomonas reinhardtii)
GPL9152 Illumina Genome Analyzer (Chlamydomonas reinhardtii)
Samples (2)
GSM829700 Chlamydomonas reinhardtii 4A+ wild-type
GSM829701 Chlamydomonas reinhardtii sor1 mutant
SRA SRP009273
BioProject PRJNA148881

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Supplementary file Size Download File type/resource
GSE33548_RAW.tar 14.4 Mb (http)(custom) TAR (of TXT)
GSE33548_readme.txt 2.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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