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Series GSE33586

Query DataSets for GSE33586

Status

Public on May 31, 2012

Title

Consequences of high temperatures and premature mortality on the transcriptome and blood physiology of wild adult sockeye salmon (Oncorhynchus nerka)

Platform organisms

Osmerus mordax; Oncorhynchus mykiss; Salmo salar; Coregonus clupeaformis; Oncorhynchus tshawytscha

Sample organism

Oncorhynchus nerka

Experiment type

Expression profiling by array

Summary

We collected sockeye salmon from the Fraser River, British Columbia, and held them at ecologically relevant temperatures (14C and 19C) determine the effect of elevated water temperature on cellular processes in non-lethally sampled gill tissue and blood plasma over a period of seven days that represents a significant portion of their upstream migration. Time-matched fish that died prematurely over the course of the holding study were also sampled for gill tissue and the transcriptomic responses in moribund fish were compared with surviving fish. This is the first study to experimentally examine transcriptomic responses to high water temperature and premature mortality in wild-caught Pacific salmon and the results will help in understanding some of the cellular mechanisms involved in large-scale migration mortality in Pacific salmon during warm water periods and for predicting or understanding causes of mortality in naturally senescing adult Pacific salmon.

Overall design

Forty samples were analyzed on forty two-channel microarrays, using a common reference design, with multiple biological replicates for each temperature condition. Fish were further classified into survivor and moribund based on their status after 7 days in captivity.

Citation(s)

Submission date

Nov 09, 2011

Last update date

May 18, 2016

Contact name

Paul Pavlidis

E-mail(s)

paul@chibi.ubc.ca

Organization name

University of British Columbia

Department

Centre for High-Throughput Biology / Psychiatry

Street address

177 Michael Smith Laboratories 2185 East Mall

City

Vancouver

State/province

British Columbia

ZIP/Postal code

V6T 1Z4

Country

Canada

Platforms (1)

GRASP 16K version 2

Samples (40)

More...

GSM830349	Adams salmon 83583 held at 14.0C, Mortality
GSM830350	Adams salmon 83584 held at 14.0C, Mortality
GSM830351	Adams salmon 83585 held at 14.0C, Mortality

GSM830352	Adams salmon 83586 held at 14.0C, Mortality
GSM830353	Adams salmon 83587 held at 19.0C, Mortality
GSM830354	Adams salmon 83588 held at 19.0C, Mortality
GSM830355	Adams salmon 83589 held at 19.0C, Mortality
GSM830356	Adams salmon 83590 held at 19.0C, Mortality
GSM830357	Adams salmon 83591 held at 19.0C, Mortality
GSM830358	Adams salmon 83592 held at 19.0C, Second
GSM830359	Adams salmon 83593 held at 19.0C, Second
GSM830360	Adams salmon 83594 held at 19.0C, Second
GSM830361	Chilko salmon 83595 held at 14.0C, Mortality
GSM830362	Chilko salmon 83596 held at 14.0C, Mortality
GSM830363	Chilko salmon 83597 held at 14.0C, Mortality
GSM830364	Chilko salmon 83598 held at 14.0C, Mortality
GSM830365	Chilko salmon 83599 held at 14.0C, Mortality
GSM830366	Chilko salmon 83600 held at 14.0C, Mortality
GSM830367	Chilko salmon 83601 held at 14.0C, Second
GSM830368	Chilko salmon 83602 held at 14.0C, Second
GSM830369	Chilko salmon 83603 held at 14.0C, Second
GSM830370	Chilko salmon 83604 held at 14.0C, Second
GSM830371	Chilko salmon 83605 held at 14.0C, Second
GSM830372	Chilko salmon 83606 held at 14.0C, Second
GSM830373	Chilko salmon 83607 held at 19.0C, Mortality
GSM830374	Chilko salmon 83608 held at 19.0C, Mortality
GSM830375	Chilko salmon 83609 held at 19.0C, Mortality
GSM830376	Chilko salmon 83610 held at 19.0C, Mortality
GSM830377	Chilko salmon 83611 held at 19.0C, Mortality
GSM830378	Chilko salmon 83612 held at 19.0C, Mortality
GSM830379	Chilko salmon 83613 held at 19.0C, Mortality
GSM830380	Chilko salmon 83614 held at 19.0C, Mortality
GSM830381	Chilko salmon 83615 held at 19.0C, Second
GSM830382	Chilko salmon 83616 held at 19.0C, Second
GSM830383	Chilko salmon 83617 held at 19.0C, Second
GSM830384	Chilko salmon 83618 held at 19.0C, Second
GSM830385	Chilko salmon 83619 held at 19.0C, Second
GSM830386	Chilko salmon 83620 held at 19.0C, Second
GSM830387	Chilko salmon 83621 held at 19.0C, Second
GSM830388	Chilko salmon 83622 held at 19.0C, Second

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE33586_RAW.tar	128.0 Mb	(http)(custom)	TAR (of TXT)
Processed data included within Sample table

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