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| Status |
Public on Oct 21, 2012 |
| Title |
A mutation in the Srrm4 gene causes pre-mRNA splicing defects and deafness in Bronx waltzer mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The spontaneous mutant Bronx waltzer (bv) mouse line is characterized by deafness and balance defect. We located the bv mutation to the Srrm4 gene which encodes a regulator of alternative pre-mRNA splicing. We found that Srrm4 is expressed in balance and hearing organs (i.e. in the vestibular maculas and the cochlea). Srrm4 is also expressed in the central nervous system including the cerebellum. To identify potential splicing defects in bv/bv mice, we analyzed RNA samples from the vestibular maculas and cerebellums of bv/bv mice and control (bv/+) littermates, using mouse exon junction microarrays (MJAY).
In this dataset, we include probe-set level data obtained from cerebellar samples. The processed data represent probe-set intensities that have been normalized to gene expression levels.
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| |
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| Overall design |
8 total samples were analyzed in this series: cerebellums from 4 heterozygous (bv/+) and 4 homozygous (bv/bv) mice at P15.
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| Contributor(s) |
Nakano Y, Banfi B |
| Citation(s) |
23055939 |
| |
| Submission date |
Nov 09, 2011 |
| Last update date |
May 18, 2016 |
| Contact name |
Botond Banfi |
| E-mail(s) |
botond-banfi@uiowa.edu
|
| Phone |
1-319-335-4228
|
| Organization name |
University of Iowa
|
| Department |
Anatomy and Cell Biology
|
| Lab |
Banfi Lab
|
| Street address |
2501 Crosspark Road
|
| City |
Coralville |
| State/province |
Iowa |
| ZIP/Postal code |
52241 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14865 |
[mjay_r2] Custom Affymetrix Mouse Exon Junction Array |
|
| Samples (8)
|
| GSM830433 |
cerebellum (bv/+) at P15, biological rep1 |
| GSM830434 |
cerebellum (bv/+) at P15, biological rep2 |
| GSM830435 |
cerebellum (bv/+) at P15, biological rep3 |
| GSM830436 |
cerebellum (bv/+) at P15, biological rep4 |
| GSM830437 |
cerebellum (bv/bv) at P15, biological rep1 |
| GSM830438 |
cerebellum (bv/bv) at P15, biological rep2 |
| GSM830439 |
cerebellum (bv/bv) at P15, biological rep3 |
| GSM830440 |
cerebellum (bv/bv) at P15, biological rep4 |
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| This SubSeries is part of SuperSeries: |
| GSE33591 |
A mutation in the Srrm4 gene causes alternative splicing defects and deafness in Bronx waltzer mice. |
|
| Relations |
| BioProject |
PRJNA154335 |
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