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Status |
Public on Jun 03, 2012 |
Title |
Streptomyces coelicolor A3(2) SigR regulon |
Organism |
Streptomyces coelicolor A3(2) |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
We determined the genomic locations of the SigR binding sites by chromatin immuno-precipitation of cross-linked DNA and hybridization on a tilling oligonucleotide array after 20 minutes of diamide treatment on cell cultures.
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Overall design |
Three independent biological samples were prepared separately from the wild-type cells and pooled before hybridization on a single array. Three more independent biological samples were prepared from sigR deletion mutant cells, pooled, and hybridized on another single array. After normalization, the signal from the mutant cells was subtracted from the signal from the wild-type cells to recover SigR specific signal.
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Contributor(s) |
Dufour YS, Kim M, Yoo J, Cho Y, Park J, Nam G, Kim H, Donohue T, Roe J |
Citation(s) |
22651816 |
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Submission date |
Nov 09, 2011 |
Last update date |
Jun 05, 2012 |
Contact name |
Yann S Dufour |
Organization name |
University of Wisconsin - Madison
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Department |
Bacteriology
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Lab |
Timothy Donohue
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Street address |
1550 Linden Drive
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platforms (1) |
GPL14825 |
UWMadison/Donohue Streptomyces coelicolor A3(2) 385K_v1.0 |
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Samples (1) |
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Relations |
BioProject |
PRJNA148535 |