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| Status |
Public on Apr 09, 2013 |
| Title |
Expression data from mouse intestine: C57Bl/6 MTHFR+/- vs BALB/c MTHFR+/- |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Previous studies in our laboratory have shown that low folate diet (control diet with 2mg folate/kg, low folate diet with 0.3mg folate/kg) can induce intestinal tumors in BALB/c mice. In addition, we reported that C57Bl/6J mice did not form tumors under the same conditions.
We used microarrays to identify the genetic differences between BALB/c and C57Bl/6J mice that promote tumorigenesis in BALB/c mice.
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| Overall design |
Two groups of mice will be used in the micro-array experiment. One group will consist of four BALB/c mice and the second group will consist of four C57Bl/6 mice. Both groups have mild MTHFR deficiency (Mthfr+/-) and will receive low folate diet (0.3 mg folate/kg) for one year. RNA samples will be extracted from duodenum, the first section of the small intestine where tumors were observed in BALB/c mice. Eight Affymetrix GeneChip Mouse Gene 1.0 ST Array Chips will be used in this experiment.
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| Contributor(s) |
Cao Y, Leclerc D |
| Citation(s) |
23001810 |
| Submission date |
Nov 29, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Daniel Leclerc |
| E-mail(s) |
daniel.leclerc@mail.mcgill.ca
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| Phone |
514-412-4400 x23281
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| Fax |
514-412-4331
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| Organization name |
Montreal Children's Hospital
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| Department |
Medical Genetics, Room 242
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| Lab |
Rima Rozen Laboratory
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| Street address |
4060 Ste-Catherine West
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3Z 2Z3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA150593 |
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