|
| Status |
Public on Apr 03, 2012 |
| Title |
BW25113 H202 and delta rpoS persister cells vs. stationary phase BW25113 wild-type |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
E. coli K-12 BW25113 persister cells generated via H202 pre-treatment and deletion of rpoS, relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL.
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| |
|
| Overall design |
Strains: BW25113 wild-type, pre-treated with H202 10 min, ampicillin 5 h; BW25113 delta rpoS, ampicillin 5 h; BW25113 wild-type OD at 600 nm of 3.0, ampicillin 30 mins.
Medium: LB
ampicillin 20 ug/mL used to generate persisters; ampicillin 5 ug/mL added to stationary phase BW25113 wild-type
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| |
|
| Contributor(s) |
O'Connor HF, Benedik M, Wood TK |
| Citation(s) |
22221537 |
| |
| Submission date |
Nov 29, 2011 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Thomas K. Wood |
| E-mail(s) |
twood@engr.psu.edu
|
| Phone |
814-863-4811
|
| Organization name |
Pennsylvania State University
|
| Department |
Chemical Engineering & Biochemistry and Molecular Biology
|
| Lab |
Dr. Wood's Lab
|
| Street address |
Room 161 Fenske Laboratory, University Park
|
| City |
State College |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (3) |
| GSM840577 |
BW25113 delta rpoS persisters T5 hrs ampicillin |
| GSM840609 |
BW25113 wild-type H202 pre-treatment 5 h ampicillin persisters |
| GSM840625 |
BW25113 wild-type stationary phase OD 3.0 |
|
| Relations |
| BioProject |
PRJNA148071 |
