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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 30, 2005 |
| Title |
Arsenic-induced changes in gene expression in the neural tube of Folr2-/- KO mouse embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to more thoroughly investigate the mechanism of arsenate-induced NTDs we designed experiment in which highly sensitive Folr2 nullizygous mice were treated i.p. with teratogenic dose of sodium arsenate just at the beginning of the neural tube formation process. This specific knockout mouse and arsenic exposure conditions were chosen as they warranted high incidence of exencephaly in exposed embryos. We investigated the gene expression changes induced by arsenic in the anterior part of neural tube in order to discover patterns that might shed light on the mechanism of arsenic’s teratogenicity.
Keywords: toxic response
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| Overall design |
On GD 7:12 and 8:12, pregnant Folr2-/- KO dams were injected ip with water solution of sodium arsenate at the dose of 9.6 mg As/ kg of body weight(treated) or with water for injection only (control). At three selected time-points: 8:15 (3 hours post-injection), 9:00 (12 hours post-injection) and 9:12 (24 hours post-injection), at least 4 control and arsenic treated dams were sacrificed and embryos harvested. From these embryos, the anterior part of neural tube was dissected and RNA extracted. No less than 6 embryonic neural tubes (3controls and 3 arsenic treated) for each time-point were used for hybridization on commercially available CodeLink UniSet 10K Bioarray. Together, 22 control and treated samples at above mentioned, 3 selected time-points were used in this study
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| Contributor(s) |
Wlodarczyk B, Cabrera R, Bozinov D, Hill D, Finnell R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 04, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Bogdan Wlodarczyk |
| E-mail(s) |
bwlodarczyk@ibt.tamhsc.edu
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| Phone |
716-677-7722
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| Fax |
713-6777784
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| Organization name |
IBT
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| Department |
CEGM
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| Lab |
Finnell's lab
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| Street address |
2121 W Holcombe Blv.
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL2909 |
CodeLink Uniset Mouse I Bioarray |
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| Samples (22)
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| GSM76937 |
Neural tube, control, GD8:15, C211680 |
| GSM76938 |
Neural tube, control, GD8:15, C216799 |
| GSM76939 |
Neural tube, control, GD8:15, C215582 |
| GSM76940 |
Neural tube, control, GD8:15, C216801 |
| GSM76941 |
Neural tube, treated, GD8:15, T216802 |
| GSM76943 |
Neural tube, treated, GD8:15, T216803 |
| GSM76944 |
Neural tube, treated, GD8:15, T216804 |
| GSM76945 |
Neural tube, treated, GD8:15, T216811 |
| GSM76946 |
Neural tube, control, GD9:00, C215570 |
| GSM76947 |
Neural tube, control, GD9:00, C215577 |
| GSM76948 |
Neural tube, control, GD9:00, C215578 |
| GSM76950 |
Neural tube, control, GD9:00, C215579 |
| GSM76951 |
Neural tube, treated, GD9:00, T215571 |
| GSM76952 |
Neural tube, treated, GD9:00, T215580 |
| GSM76953 |
Neural tube, treated, GD9:00, T215581 |
| GSM76954 |
Neural tube, control, GD9:12, C214260 |
| GSM76955 |
Neural tube, control, GD9:12, C215583 |
| GSM76956 |
Neural tube, control, GD9:12, C215584 |
| GSM76957 |
Neural tube, treated, GD9:12, T215585 |
| GSM76959 |
Neural tube, treated, GD9:12, T215586 |
| GSM76961 |
Neural tube, treated, GD9:12, T215587 |
| GSM76962 |
Neural tube, treated, GD9:12, T215588 |
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| Relations |
| BioProject |
PRJNA93435 |
| Supplementary data files not provided |
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