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Series GSE34303 Query DataSets for GSE34303
Status Public on Nov 21, 2013
Title A Global Transcriptome Analysis of BMSC Senescence
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Bone marrow stromal cells (BMSCs) can be expanded by serial passage, but expansion is limited by cell senescence.  The nature of changes associated with BMSC serial passages was assessed. Transcriptome analysis of 10 early and 15 late passage samples from 5 subjects revealed 2193 differentially expressed genes; those highly expressed in early passage cells were overrepresented in skeletal system development, embryonic morphogenesis, tube morphogenesis, etc, while those highly expressed in the late passage BMSCs were overrepresented in nucleosome assembly; chromatin assembly, DNA packaging, etc. 57 BMSC samples from 7 donors were further analyzed for the transition from an early to late passage; 155 genes were highly correlated with BMSC senescence and a set of 24 genes was predictive of BMSCs lifespan. The change from an early to a late passage molecular signature occurred between passage 3 and 5. In contrast, senescence associated beta-galactosidase staining began to increase after passage 6 or 7 and colony formation efficiency began to fall after passage 7. These data indicated that the onset of molecular changes associated with BMSC passage varied among individuals and preceded changes in commonly used indicators of BMSC senescence. The set of 24 BMSC lifespan predictive genes will be useful in assessing the quality of clinical BMSC products.
 
Overall design We performed gene expression profiling on serial passages of BMSCs from 7 healthy donors (09FC20, 09FC37, 09FC43, 09FC44, 09FC45, 09FC49, and W10003).
 
Citation(s) 23959330
Submission date Dec 09, 2011
Last update date Feb 22, 2018
Contact name Francesco Maria Marincola
E-mail(s) fmarincola@sidra.org
Phone 301-793-8210
Organization name Sidra Medical and Research Center
Street address Al Nasr Tower, AL Corniche Street, PO Box 26999
City Doha
ZIP/Postal code PO Box 26999
Country Qatar
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (57)
GSM846967 Donor 6 - Passage 3 - mAdbID:107843
GSM846968 Donor 6 - Passage 8 - mAdbID:107955
GSM846969 Donor 6 - Passage 7 - mAdbID:107956
Relations
BioProject PRJNA151525

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34303_RAW.tar 811.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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