|
| Status |
Public on Apr 25, 2012 |
| Title |
Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The transcriptome of the planarian Schmidtea mediterranea is not well characterized. We have used RNA-Seq to characterize the transcriptome in both sexual and asexual strains of S. mediterranea from both untreated and irradiated animals. Moreover, we have performed RNA-Seq on RNA purified from FACS sorted neoblasts and differentiated cells. Together these studies expand our understanding of the planarian transcriptome and have identified strain-specific, neoblast-specific, and conserved transcripts.
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| Overall design |
RNA-Seq was performed on RNA isolated from untreated and irradiated S. mediterranea animals from both the sexual and asexual strains, on FACS purified X1 neoblasts, X2 neoblasts, and Xins differentiated cells.
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| |
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| Contributor(s) |
Resch AM, Palakodeti D, Lu Y, Graveley BR |
| Citation(s) |
22496805 |
| |
| Submission date |
Dec 09, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Brenton R Graveley |
| E-mail(s) |
graveley@uchc.edu
|
| Phone |
860-679-2090
|
| Organization name |
University of Connecticut Health Center
|
| Department |
Genetics and Genome Sciences
|
| Street address |
400 Farmington Avenue
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030-3301 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14991 |
Illumina Genome Analyzer IIx (Schmidtea mediterranea) |
|
| Samples (9)
|
| GSM847464 |
Sexual Irradiated |
| GSM847465 |
Sexual Non-irradiated [single end] |
| GSM847466 |
Sexual Non-irradiated [paired end] |
| GSM847467 |
Sexual Non-irradiated, X1 FACS purified neoblast |
| GSM847468 |
Sexual Non-irradiated, Xins FACS purified differentiated cells |
| GSM847469 |
Sexual Non-irradiated, X2 FACS purified neoblast |
|
| Relations |
| SRA |
SRP009681 |
| BioProject |
PRJNA151483 |
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