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| Status |
Public on Dec 16, 2012 |
| Title |
Gene expression analysis of wild type and KAP1 KO mouse T cell progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The modulation of chromatin status at specific genomic loci controls lymphoid differentiation. Here, we investigated the role played in this process by KAP1, the universal cofactor of KRAB-containing Zinc Finger Proteins (KRAB-ZFPs), a tetrapod-restricted family of transcriptional repressors. T cell-specific Kap1 knockout mice displayed a significant expansion of immature thymocytes and imbalances in the ratios of mature T cells in the thymus and the spleen, with impaired responses to TCR stimulation. Transcriptome and chromatin studies revealed that KAP1 directly controls the expression of a number of genes involved in TCR and cytokine signalling, among which Traf1 and FoxO1, and is strongly associated with cis-acting regulatory elements marked by the H3K9me3 repressive mark on the genome of thymic T cells. Likely responsible for tethering KAP1 to at least part of its genomic targets, a small number of KRAB/ZFPs are selectively expressed in T lymphoid cells. These results reveal the so far unsuspected yet important role of KRAB/KAP1-mediated epigenetic regulation in T lymphocyte differentiation and activation.
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| |
|
| Overall design |
Cells were harvested form the thymus of 3 CD4-Cre/Kap1flox (KAP1 KO in the T lineage) mice and 3 wt/Kap1flox littermate controls
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| |
|
| Contributor(s) |
Santoni de Sio FR, Kapopoulou A, Trono D |
| Citation(s) |
22872677 |
| |
| Submission date |
Dec 14, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Francesca R. Santoni de Sio |
| E-mail(s) |
santonidesio.francesca@hsr.it
|
| Organization name |
San Raffaele Scientific Institute
|
| Department |
SR-TIGET
|
| Lab |
Mechanisms of Peripheral Tolerance
|
| Street address |
via Olgettina 58
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE38580 |
KAP1 regulates gene networks controlling T cell development and activation |
|
| Relations |
| BioProject |
PRJNA151207 |
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