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| Status |
Public on Jan 10, 2013 |
| Title |
Three acyltransferases and a nitrogen responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas |
| Organism |
Chlamydomonas reinhardtii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Three acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG accumulation based on their patterns of expression. Each gene also shows increased mRNA abundance in other TAG-accumulating conditions (-S, -P, -Zn, -Fe). Insertional mutants, pdat1-1 and pdat1-2, accumulate 25% less TAG compared to the parent strain, D66+, which demonstrates the relevance of the trans-acylation pathway in Chlamydomonas. The biochemical functions of DGTT1 and PDAT1 were validated by rescue of oleic acid sensitivity and restoration of TAG accumulation in a yeast strain lacking all acyltransferase activity. Time course analysis suggest than an SBP domain transcription factor protein, whose mRNA increases precede that of other genes like DGAT1, is a candidate regulator of the N-deficiency response. An insertional mutant, nrr1-1, accumulates only 50% of the TAG compared to the parental strain in N-starvation conditions and is unaffected by other nutrient stresses, suggesting the operation of multiple signaling pathways leading to stress-induced TAG accumulation.
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| Overall design |
Sampling of Chlamydomonas 2137 cultivated in TAP, N-Free TAP or TAP with varying amounts of N.
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| Contributor(s) |
Boyle NR, Page MD, Liu B, Blaby IK, Casero D, Kropat J, Cokus S, HongHermesdorf A, Shaw J, Karpowicz SJ, Johnson S, Grossman A, Benning C, Pellegrini M, Merchant SS |
| Citation(s) |
22403401 |
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| Submission date |
Dec 20, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Sabeeha Merchant |
| E-mail(s) |
merchant@chem.ucla.edu
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| Phone |
310-825-8300
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| Organization name |
University of California Los Angeles
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| Department |
Department of Chemistry and Biochemistry
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| Street address |
607 Charles E. Young Drive East
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-1569 |
| Country |
USA |
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| Platforms (1) |
| GPL9100 |
Illumina Genome Analyzer II (Chlamydomonas reinhardtii) |
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| Samples (30)
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| GSM851599 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=0 |
| GSM851600 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=0' |
| GSM851601 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=2m |
| GSM851602 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=4m |
| GSM851603 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=8m |
| GSM851604 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=12m |
| GSM851605 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=18m |
| GSM851606 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=24m |
| GSM851607 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=30m |
| GSM851608 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=45m |
| GSM851609 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency short time course - t=60m |
| GSM851610 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=0 |
| GSM851611 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=0 (2) |
| GSM851612 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=30m |
| GSM851613 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=30m (2) |
| GSM851614 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=4h |
| GSM851615 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=4h (2) |
| GSM851616 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=8h |
| GSM851617 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency first long time course - t=8h (2) |
| GSM851618 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=0h |
| GSM851619 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=2h |
| GSM851620 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=8h |
| GSM851621 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=12h |
| GSM851622 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=24h |
| GSM851623 |
Chlamydomonas reinhardtii 2137 - Nitrogen deficiency second long time course - t=48h |
| GSM851624 |
Chlamydomonas reinhardtii 2137 - NH4Cl supplementation experiment - [NH4Cl]=0.35mM |
| GSM851625 |
Chlamydomonas reinhardtii 2137 - NH4Cl supplementation experiment - [NH4Cl]=0.7mM |
| GSM851626 |
Chlamydomonas reinhardtii 2137 - NH4Cl supplementation experiment - [NH4Cl]=1.75mM |
| GSM851627 |
Chlamydomonas reinhardtii 2137 - NH4Cl supplementation experiment - [NH4Cl]=3mM |
| GSM851628 |
Chlamydomonas reinhardtii 2137 - NH4Cl supplementation experiment - [NH4Cl]=7mM |
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| Relations |
| SRA |
SRP010062 |
| BioProject |
PRJNA150503 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34585_RAW.tar |
9.7 Gb |
(http)(custom) |
TAR (of BOWTIE) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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