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| Status |
Public on Nov 01, 2012 |
| Title |
Transcriptome response to genistein in zebrafish embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
To test wether endocrine disruption is detectable in zebrafish at the transcriptome level, we exposed newly fertilized zebrafish embryos to low doses of genistein (EC10 and EC20) for 48 hours.
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| Overall design |
Newly fertilized embryos (<2hpf) were exposed to 2.2 mg/l (EC10) and 2.4 mg/l (EC20) genistein for 48 hours. Since aceton was used as a solvent for genistein, an acetone solvent control in addition to the water (ISO) control was included in the study. Overall, four replicates were performed per condition, each consisting of 24 pooled embryos.
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| Contributor(s) |
Schiller V, Wichmann A, Kriehuber R, Hecker M, Fenske M |
| Citation(s) |
23017276 |
| |
| Submission date |
Dec 21, 2011 |
| Last update date |
Feb 05, 2013 |
| Contact name |
Viktoria Schiller |
| E-mail(s) |
schiller@molbiotech.rwth-aachen.de
|
| Phone |
0049 (0)241 6085 12272
|
| Organization name |
Fraunhofer IME
|
| Department |
UNIFISH
|
| Street address |
Forckenbeckstrasse 6
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6457 |
Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Feature Number version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA150451 |
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