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Series GSE34803 Query DataSets for GSE34803
Status Public on Mar 05, 2013
Title Expression data of Nodule Cysteine-Rich (NCR) Defensin-Like (DEFL) genes in different stages of nodule development in Medicago truncatula
Organism Medicago truncatula
Experiment type Expression profiling by array
Summary We have undertaken a detailed study to identify mechanisms regulating expression of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in different stages of nodule development. Additionally, rhizobial mutants were used to understand the importance of the rhizobial components in induction of NCRs. Early NCRs were detected during the initial infection of rhizobia in nodules and continue to be expressed into the late stages of nodule development. Late NCRs were induced concomittant with bacteroid development in the nodules. The induction of these groups of genes was correlated with the number and morphology of rhizobia in the nodule.
 
Overall design We used a custom Affymetrix chip containing 684 probe sequences of Medicago DEFLs to explore the expression patterns of NCRs in nodules inoculated with Sinorhizobium meliloti 1021(Sm1021) at marked developmental stages and nodules inoculated with various mutants derived from Sm1021 totalling 14 different treatments. Each treatment was supported by three biological replicates giving a grand total of 42 samples.
 
Contributor(s) Silverstein KA, Nallu S, VandenBosch KA, Samac DA, Bucciarelli B, Vance C
Citation(s) 23573247
Submission date Jan 03, 2012
Last update date May 31, 2013
Contact name Kevin A Silverstein
Organization name Universtiy of Minnesota
Department Minnesota Supercomuting Institute
Street address 599 Walter Library, 117 Pleasant St SE
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL14988 UMN MtDEFL 1.0
Samples (42)
GSM847141 Mock roots-14dpi-biological rep1
GSM847142 Mock roots-14dpi-biological rep2
GSM847143 Mock roots-14dpi-biological rep3
Relations
BioProject PRJNA151133

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34803_RAW.tar 6.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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