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| Status |
Public on Apr 01, 2012 |
| Title |
Gene expression profiles of tumor-associated macrophages (TAMs) overexpressing miR-511-3p |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Hematopoietic stem/progenitor cells (HS/PCs) were transduced with lentiviral vectors overexpressing OFP and either miR-511-3p or a control, mutated miRNA sequence (miR-511-3p-mut). The transduced HS/PCs were then transplanted in recipient C57BL/6 mice. Tumors (Lewis lung carcinomas, LLC) were injected s.c. 4 weeks after HS/PC transplant. Lentiviral vector-transduced (OFP+), tumor-associated macrophages (TAMs) were isolated 4 weeks after LLC injection by fluorescence-activated cell sorting. Gene expression profiles of TAMs overexpressing either miR-511-3p or miR-511-3p-mut were obtained from 3 independent biological samples/each. Gene expression profiles of miRNA-overexpressing TAMs were then compared with the gene expression profiles of wild-type TAMs isolated from LLCs grown in nontransplanted C57BL/6 mice; the latter TAMs were subfractioned into MRC1+CD11c(low) or CD11c+MRC1(low) subsets before RNA isolation and analysis. Comparison of gene expression profiles of TAMs revealed that miR-511-3p overexpression tunes down the expression of multiple genes that are classically upregulated in protumoral MRC1+CD11c(low) TAMs.
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| Overall design |
TAMs were isolated from Lewis lung carcinomas grown s.c. in C57BL/6 mice.
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| Contributor(s) |
Squadrito ML, De Palma M, Lyle R, Gilfillan GD |
| Citation(s) |
22832163 |
| |
| Submission date |
Jan 06, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Camilla Stormo |
| E-mail(s) |
Camilla.Stormo@medisin.uio.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Medical Genetics
|
| Street address |
Kirkeveien 166
|
| City |
Oslo |
| ZIP/Postal code |
0407 |
| Country |
Norway |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| SRA |
SRP010190 |
| BioProject |
PRJNA150173 |
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