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| Status |
Public on Jan 07, 2012 |
| Title |
Human colon cancer HT-29 and LS174T cells: stably transfected with shRNA-Ascl2/EGFP vs. stably transfected with shRNA-Control/EGFP |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Achaete scute-like 2 (Ascl2), a basic helix-loop-helix (bHLH) transcription factor, controls the fate of intestinal stem cells. However, the role of Ascl2 in colon cancer progenitor cells remains unknown. The cell lines HT-29 (47.5-95% of CD133+ population) and LS174T (0.45% of CD133+ population) were chosen for functional evaluation of Ascl2 in colon cancer progenitor cells after gene knockdown by RNA interference. The microRNA (miRNA) microarrays identified 26 two-fold up-regulated miRNAs and 58 two-fold down-regulated miRNAs in shRNA-Ascl2/HT-29 cells and 178 two-fold up-regulated miRNAs and 172 two-fold down-regulated miRNAs in shRNA-Ascl2/LS174T cells.
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| Overall design |
Two-condition experiment: shRNA-Ascl2/HT-29 cells vs. shRNA-Ctr/HT-29 cells, and shRNA-Ascl2/LS174T cells vs. shRNA-Ctr/LS174T cells. Biological replicates: 1 HT-29 cells stably transfected with shRNA-Ascl2/EGFP, 1 LS174T cells stably transfected with shRNA-Ascl2/EGFP, 1 HT-29 cells stably transfected with shRNA-Control/EGFP, and 1 LS174T cells stably transfected with shRNA-Control/EGFP, independently grown and harvested. One replicate per array.
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| Contributor(s) |
Wang R, Zhu R |
| Citation(s) |
22384170, 25371200 |
| Submission date |
Jan 06, 2012 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Rongquan Wang |
| E-mail(s) |
rongquanw@hotmail.com
|
| Phone |
86-23-68754124
|
| Fax |
86-23-65410853
|
| Organization name |
Southwest Hospital
|
| Department |
Department of Gastroenterology
|
| Street address |
Gaotanyan Main Street 30#
|
| City |
Chongqing |
| State/province |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11434 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno |
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| Samples (4)
|
| GSM857541 |
shRNA-Ascl2/EGFP-transfected HT-29 cells |
| GSM857542 |
shRNA-Control/EGFP-transfected HT-29 cells |
| GSM857543 |
shRNA-Ascl2/EGFP-transfected LS174T cells |
| GSM857544 |
shRNA-Control/EGFP-transfected LS174T cells |
|
| Relations |
| BioProject |
PRJNA150153 |
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