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| Status |
Public on Jul 24, 2016 |
| Title |
Whole-Genome Transcriptome Profiling of Microvascular Free Flaps |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Vascular compromise in microsurgical free flap transfers continues to present devastating outcomes in terms of flap failure and increased operating room costs. Current devices to detect early venous congestion have not become widely accepted because of insufficient sensitivity, incisiveness, or practicality.
In our study, we developed a venous obstruction rat model in which the superficial inferior epigastric (SIE) vessels to the lower abdominal flaps was separated and artery or vein was ligated, and the flap reattached. Flaps were harvested 4 hours post-op. Total RNA was isolated and gene expression profiles were compared by Affymetrix Whole Genome 230 2.0.
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| Overall design |
Flaps from groups of Venous or artery congestion (VC or AC, resp.) and sham operation group, were harvested and RNA extraction and hybridization on Affymetrix microarrays. Other controls including histology assays to assure comparable phenotype within groups.
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| Contributor(s) |
Zhang Y, Nguyen G, Hwang B |
| Citation(s) |
23977093 |
| Submission date |
Jan 23, 2012 |
| Last update date |
Oct 25, 2019 |
| Contact name |
Yiqiang Zhang |
| Organization name |
University of Hawaii Manoa
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| Department |
Anatomy, Biochemistry and Physiology
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| Lab |
Zhang Lab
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| Street address |
651 Ilalo St BSB-311D
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| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96813 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (14)
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| GSM864807 |
Control, biological rep5 |
| GSM864808 |
Venous constriction, biological rep1 |
| GSM864809 |
Venous constriction, biological rep2 |
| GSM864810 |
Venous constriction, biological rep3 |
| GSM864811 |
Venous constriction, biological rep4 |
| GSM864812 |
Venous constriction, biological rep5 |
| GSM864813 |
Artery constriction, biological rep1 |
| GSM864814 |
Artery constriction, biological rep2 |
| GSM864815 |
Artery constriction, biological rep3 |
| GSM864816 |
Artery constriction, biological rep4 |
| GSM864817 |
Artery constriction, biological rep5 |
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| Relations |
| BioProject |
PRJNA152845 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35270_RAW.tar |
38.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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