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| Status |
Public on Jan 09, 2014 |
| Title |
GSK3B shRNA in Panc-1 xenografts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The Panc-1 human pancreatic cancer line (ATCC) was constructed to stably express control or GSK3B shRNA. Control or GSK3B shRNA expressing Panc-1 cells were implanted subcutaneously into the flanks of 3-5 week old, female, athymic nude mice. Tumors were grown to at least 250mm3. Tumor tissue was microdissected for further analysis. We compared control shRNA (n=4) to GSK3B shRNA (n=3) Panc-1 xenografts. The array data with simple statistical calculations are also provided in a supplementary Excel workbook, with probe-set annotation that we used at the time (users may want newer annotation).
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| Overall design |
We compared xenografts of Panc-1 (Human pancreatic carcinoma cell line, grown in mice) stably expressing GSK3B shRNA (n=3) to similar xenografts with control shRNA (n=4). Xenografts were grown in the flanks of female, athymic nude mice until they reached at least 250mm3. Tumor tissue was microdissected for further analysis. mRNA abundance assays were performed using Affymetrix HG_U133_plus_2 arrays, with 54675 probe-sets.
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| Contributor(s) |
Morgan M, Bhojani MS, Kuick R, Spalding A, Ben-Josef E |
| Citation(s) |
24944842 |
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Zhang Q, Bhojani MS, Ben-Josef E, Spalding AC, Kuick R, Sun Y, and Morgan MA. Glycogen Synthase Kinase 3β in Pancreatic Cancer and its Implications in Chemotherapy and Radiation Therapy. J Carcinogene Mutagene, 2013, 4:147. doi: 10.4172/2157-2518.1000147
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| Submission date |
Jan 26, 2012 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Rork Kuick |
| E-mail(s) |
rork@umich.edu
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| Phone |
734-936-9241
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| Organization name |
University of Michigan
|
| Street address |
University of Michigan, SPH II, 1415 Washington Heights, Room M2533
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2029 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA152729 |
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