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Series GSE35351 Query DataSets for GSE35351
Status Public on Jan 09, 2014
Title GSK3B shRNA in Panc-1 xenografts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The Panc-1 human pancreatic cancer line (ATCC) was constructed to stably express control or GSK3B shRNA. Control or GSK3B shRNA expressing Panc-1 cells were implanted subcutaneously into the flanks of 3-5 week old, female, athymic nude mice. Tumors were grown to at least 250mm3. Tumor tissue was microdissected for further analysis. We compared control shRNA (n=4) to GSK3B shRNA (n=3) Panc-1 xenografts. The array data with simple statistical calculations are also provided in a supplementary Excel workbook, with probe-set annotation that we used at the time (users may want newer annotation).
Overall design We compared xenografts of Panc-1 (Human pancreatic carcinoma cell line, grown in mice) stably expressing GSK3B shRNA (n=3) to similar xenografts with control shRNA (n=4). Xenografts were grown in the flanks of female, athymic nude mice until they reached at least 250mm3. Tumor tissue was microdissected for further analysis. mRNA abundance assays were performed using Affymetrix HG_U133_plus_2 arrays, with 54675 probe-sets.
Contributor(s) Morgan M, Bhojani MS, Kuick R, Spalding A, Ben-Josef E
Citation(s) 24944842
Zhang Q, Bhojani MS, Ben-Josef E, Spalding AC, Kuick R, Sun Y, and Morgan MA. Glycogen Synthase Kinase 3β in Pancreatic Cancer and its Implications in Chemotherapy and Radiation Therapy. J Carcinogene Mutagene, 2013, 4:147. doi: 10.4172/2157-2518.1000147
Submission date Jan 26, 2012
Last update date Aug 01, 2019
Contact name Rork Kuick
Phone 734-936-9241
Organization name University of Michigan
Street address University of Michigan, SPH II, 1415 Washington Heights, Room M2533
City Ann Arbor
State/province MI
ZIP/Postal code 48109-2029
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (7)
GSM866688 control shRNA replicate 1
GSM866689 control shRNA replicate 2
GSM866690 control shRNA replicate 3
BioProject PRJNA152729

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35351_GSK3B_supplement.xlsx.gz 17.1 Mb (ftp)(http) XLSX
GSE35351_RAW.tar 34.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

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