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Status |
Public on Nov 04, 2005 |
Title |
Microarray Analysis of Retinal Gene Expression in the DBA/2J Model of Glaucoma |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months. Results: There were changes in expression of 68 genes, with 32 genes increasing and 36 genes decreasing at 8 months versus 3 months. Upregulated genes were associated with immune response, glial activation, signaling and gene expression, while down-regulated genes included multiple crystallin genes. Significant changes in 9 upregulated genes and 2 downregulated genes were confirmed by quantitative RT-PCR, with some showing changes in expression by 5 months. Conclusions: DBA/2J retina shows evidence for glial activation and an immune-related response following IOP elevation, similar to what has been reported following acute elevation of IOP in other models. Keywords: retina, glaucoma, DBA/2J, elevated intraocular pressure
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Overall design |
IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months.
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Contributor(s) |
Steele MR, Inman DM, Calkins DJ, Horner PJ, Vetter ML |
Citation(s) |
16505032 |
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Submission date |
Nov 03, 2005 |
Last update date |
Feb 11, 2019 |
Contact name |
Monica L. Vetter |
E-mail(s) |
monica@neuro.utah.edu
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Phone |
(801) 581-4984
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Organization name |
University of Utah
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Department |
Neurobiology & Anatomy
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Lab |
Vetter Lab
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Street address |
20 South, 2030 East Rm. 320 BPRB
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City |
Salt Lake City |
State/province |
UT |
ZIP/Postal code |
84112-9458 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (6)
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Relations |
BioProject |
PRJNA93629 |
Supplementary file |
Size |
Download |
File type/resource |
GSE3554_RAW.tar |
34.0 Mb |
(http)(custom) |
TAR (of CEL) |
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