|
| Status |
Public on Jul 01, 2012 |
| Title |
Functional inactivation of Thyroid Transcription Factor-1 in PCCl3 thyroid cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Thyroid Transcription Factor-1 (TTF-1) is a key regulator of thyroid development and function. In order to identify the genes whose expression depends on TTF-1 transcriptional activity within the thyrocyte we analyzed the consequence of the functional inactivation of this factor in PCCl3 cells.
The expression of a fusion protein composed of the DNA binding domain of TTF-1 and of the strong repressive domain of the Engrailed protein (Engr HD) resulted in a dramatic loss of epithelial cell morphology and in proliferation arrest. These changes were reversed when the inhibition of endogenous TTF-1 was relieved. No change was observed when a similar fusion protein containing point mutations abolishing DNA binding activity (Engr HDm) was produced in the cells.
|
| |
|
| Overall design |
Microarrays hybridizations were performed on PCCl3 cells expressing a TTF1 antagonist (Engr HD –Dox) versus non-expressing cells (Engr HD +Dox), and on cells expressing the control protein (Engr HDm – Dox) versus non-expressing cells (Engr HDm + Dox).
After amplification and labelling, sample pairs were hybridized onto Rat MI ReadyArray (Microarrays Inc., Alabama USA). The oligonucleotides set consists of 34,717 70-mer transcript probes representing genes and alternative splice products from the Operon Biotechnologies' Array Ready Oligo Set version 3.0 for Rattus norvegicus. Hybridizations were replicated with dye swap.
|
| |
|
| Contributor(s) |
Christophe-Hobertus C, Lefort A, Libert F, Christophe D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2012 |
| Last update date |
Jul 03, 2012 |
| Contact name |
Frederick Libert |
| E-mail(s) |
flibert@ulb.ac.be
|
| Organization name |
ULB
|
| Department |
IRIBHM
|
| Lab |
C2-140
|
| Street address |
808 route de Lennik
|
| City |
Brussels |
| ZIP/Postal code |
B-1070 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL15166 |
Microarrays Inc. Rat RN1100 MI Ready Array |
|
| Samples (4)
|
| GSM871718 |
Rat PCCl3 thyroid cells expressing Engr HD (-Dox) vs non-expressing cells (+Dox)_1 |
| GSM871742 |
Rat PCCl3 thyroid cells expressing Engr HD (-Dox) vs non-expressing cells (+Dox)_2 |
| GSM871743 |
Rat PCCl3 thyroid cells expressing Engr HDm (-Dox) vs non-expressing cells (+Dox)_1 |
| GSM871744 |
Rat PCCl3 thyroid cells expressing Engr HDm (-Dox) vs non-expressing cells (+Dox)_2 |
|
| Relations |
| BioProject |
PRJNA152463 |
Less...
More...