|
| Status |
Public on Jul 30, 2012 |
| Title |
Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report ChIP-Seq data for GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential. We introduced HA-tagged GATA-1 or V205G into G1ME cells via retroviral transduction. The cells were crosslinked at 48h post-transduction, and an HA antibody was used for chromatin immunoprecipitation (ChIP). ChIP and input samples were sequenced on Illumina GAII high-throughput sequencer. The data reveal GATA-1-specific and V205G-specific bidning sites, indicating that FOG-1 both faacilitates and prohibits GATA-1 chromatin occupancy in a context-dependent manner.
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| |
|
| Overall design |
Examinaton of chromatin occupancy of GATA-1 anda FOG-binding mutant of GATA-1 in G1ME cells cultured in TPO.
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| Contributor(s) |
Chlon T, Dore L, Crispino J |
| Citation(s) |
22771118 |
| |
| Submission date |
Feb 09, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Timothy Michael Chlon |
| E-mail(s) |
tchlon@u.northwestern.edu, timothy.chlon@cchmc.org, tchlon@gmail.com
|
| Organization name |
Cincinnati Children's
|
| Department |
Oncology
|
| Lab |
Wells
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE35708 |
Cofactor-mediated Restriction of GATA-1 Chromatin Occupancy Coordinates Lineage-specific Gene Expression |
|
| Relations |
| SRA |
SRP010853 |
| BioProject |
PRJNA155881 |
