|
| Status |
Public on Nov 29, 2012 |
| Title |
Zebrafish ectonucleoside triphosphate/diphosphohydrolase 5 (entpd5) mutants compared to siblings at 7 dpf |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
This microarray was performed in attempt to find genes that are related to the presence of entpd5 during embryonic development
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| |
|
| Overall design |
Embryos were raised in standard E3 medium at 28 degrees Celcius. At 6 dpf mutant were separated from siblings by an in vivo skeletal staining (incubating embryos 5 minutes in 0.05% alizarin red in E3 medium where after they are extensively washed with E3 medium) as mutants fail to mineralize their skeleton. At 7 dpf, total RNA from (n=30) entpd5 mutants versus sibling embryos was isolated using the Rnaesy mini kit (Qiagen) according to the manufactures protocol.
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| |
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| Contributor(s) |
Schulte-Merker S, Logister I |
| Citation(s) |
23236130 |
| |
| Submission date |
Feb 13, 2012 |
| Last update date |
Jan 04, 2013 |
| Contact name |
Leonie Huitema |
| E-mail(s) |
l.huitema@hubrecht.eu
|
| Phone |
+3130 212 1920
|
| Fax |
+3130 251 64 64
|
| URL |
http://www.hubrecht.eu/research/schulte-merker/index.html
|
| Organization name |
Hubrecht Institute
|
| Department |
Schulte-Merker group
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL6457 |
Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Feature Number version) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA152143 |
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