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| Status |
Public on Feb 24, 2012 |
| Title |
Use of microarray analyses to determine the effect of hyperoxia and hypoxia on gene expression in early oxygen-induced retinopathy (OIR). |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Different inbred strains of rats differ in their susceptibility to OIR, an animal model of human retinopathy of prematurity. We examined gene expression profiles in Fischer 344 (F344, resistant to OIR) and Sprague Dawley (SD, susceptible to OIR) rats at the early time points of day 5 (in response to hyperoxia) and day 6 (in response to relative hypoxia) to identify gene pathways related to the underlying genetic cause of the phenotypic differences observed between strains.
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| Overall design |
To examine gene expression changes in rat strains which are resistant and susceptible to OIR, four different experimental conditions were analysed: F344 cyclic hyperoxia (O2) exposed, F344 room air (RA) exposed, SD O2 exposed and SD RA exposed. Two samples of pooled RNA, comprising of 3 individual rats from 2 separate litters, was used for each experimental condition, for each time point of interest. Pooled RNA from age-matched room air-exposed rats were used as controls.
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| Contributor(s) |
Tea M, Fogarty R, Brereton HM, Michael MZ, Kenyon R, Van der Hoek MB, Tsykin A, Coster DJ, Williams KA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 23, 2012 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Melinda N Tea |
| E-mail(s) |
melinda.tea@flinders.edu.au
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| Organization name |
Flinders University
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| Department |
Ophthalmology
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| Lab |
Rm 5D214
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| Street address |
Flinders Medical Centre, Carpark Building
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| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5042 |
| Country |
Australia |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| GSM879541 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 5 F344 rats, biological replicate 1 |
| GSM879542 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 5 F344 rats, biological replicate 2 |
| GSM879543 |
Pooled retinal RNA from room air-exposed post-natal day 5 F344 rats, biological replicate 1 |
| GSM879544 |
Pooled retinal RNA from room air-exposed post-natal day 5 F344 rats, biological replicate 2 |
| GSM879545 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 5 SD rats, biological replicate 1 |
| GSM879546 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 5 SD rats, biological replicate 2 |
| GSM879547 |
Pooled retinal RNA from room air-exposed post-natal day 5 SD rats, biological replicate 1 |
| GSM879548 |
Pooled retinal RNA from room air-exposed post-natal day 5 SD rats, biological replicate 2 |
| GSM879549 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 6 F344 rats, biological replicate 1 |
| GSM879550 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 6 F344 rats, biological replicate 2 |
| GSM879551 |
Pooled retinal RNA from room air-exposed post-natal day 6 F344 rats, biological replicate 1 |
| GSM879552 |
Pooled retinal RNA from room air-exposed post-natal day 6 F344 rats, biological replicate 2 |
| GSM879553 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 6 SD rats, biological replicate 1 |
| GSM879554 |
Pooled retinal RNA from cyclic hyperoxia-exposed post-natal day 6 SD rats, biological replicate 2 |
| GSM879555 |
Pooled retinal RNA from room air-exposed post-natal day 6 SD rats, biological replicate 1 |
| GSM879556 |
Pooled retinal RNA from room air-exposed post-natal day 6 SD rats, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA151759 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36037_RAW.tar |
63.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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