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Status |
Public on Apr 03, 2012 |
Title |
Microarray analysis to identify genes that are differentially expressed in resting and in LPS-activated splenic B cells of Ebf1+/fl-RERTCre and Ebf1fl/fl-RERTCre mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Using resting and LPS-stimulated B cells from mice carrying two Ebf1 alleles flanked with loxP sites and an inducible Cre recombinase, target genes of Ebf 1 and the effect of Ebf1 deletion on the cells was analyzed. Target genes of Ebf 1 and the effect of Ebf1 deletion on the cells was analyzed.
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Overall design |
Ebf1 fl/fl mice and heterozygote control animals carrying an ER-Cre fusion protein were treated with Tamoxifen-Citrate and were sacrificed for analysis. Resting B cells were sorted and were either left untreated or were stimulated with LPS, followed by RNA extraction for expression analysis. See related Series GSE35915.
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Contributor(s) |
Györy I, Grosschedl R |
Citation(s) |
22431510 |
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Submission date |
Feb 24, 2012 |
Last update date |
Jan 12, 2017 |
Contact name |
Soeren Boller |
E-mail(s) |
boller@ie-freiburg.mpg.de
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Phone |
0049(0)7615108755
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Organization name |
Max Planck Institute of Immunobiology and Epigenetics
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Department |
Cellular & Molecular Immunology
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Lab |
Grosschedl lab
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Street address |
Stuebeweg 51
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City |
Freiburg |
ZIP/Postal code |
79108 |
Country |
Germany |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (12)
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Relations |
Affiliated with |
GSE35915 |
BioProject |
PRJNA151811 |