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| Status |
Public on Oct 02, 2012 |
| Title |
Dual RpoH sigma factors in Sinorhizobium meliloti |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
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| Summary |
Sinorhizobium meliloti can live as a soil saprophyte, and can engage in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including two putative RpoH (heat shock) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti rpoH1, rpoH2 and rpoH1 rpoH2 mutants during heat shock and stationary phase growth. Under these conditions, expression of over 300 genes is dependent on rpoH1 and rpoH2.
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| Overall design |
Gene expression profiling of Sinorhizobium meliloti Rm1021 and its isogenic rpoH1, rpoH2, and rpoH1rpoH2 mutants, subjected to heat shock or stationary phase growth, was performed using custom Affymetrix GeneChips
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| Contributor(s) |
Barnett MJ, Bittner AN, Toman CJ, Oke V, Long SR |
| Citation(s) |
22773790 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM093628 |
Control of Symbiotic Gene Expression in Sinorhizobium meliloti |
STANFORD UNIVERSITY |
SHARON R LONG |
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| Submission date |
Mar 01, 2012 |
| Last update date |
Feb 17, 2015 |
| Contact name |
Melanie J Barnett |
| Organization name |
Stanford University
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| Department |
Biology
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| Lab |
Sharon R. Long
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| Street address |
371 Jane Stanford Way
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
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| Samples (27)
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| GSM883373 |
Sinorhizobium meliloti Rm1021, no heat shock, biological rep1 |
| GSM883374 |
Sinorhizobium meliloti Rm1021, no heat shock, biological rep2 |
| GSM883375 |
Sinorhizobium meliloti Rm1021, no heat shock, biological rep3 |
| GSM883376 |
Sinorhizobium meliloti Rm1021, heat shock, biological rep1 |
| GSM883377 |
Sinorhizobium meliloti Rm1021, heat shock, biological rep2 |
| GSM883378 |
Sinorhizobium meliloti Rm1021, heat shock, biological rep3 |
| GSM883379 |
Sinorhizobium meliloti VO3128, heat shock, biological rep1 |
| GSM883380 |
Sinorhizobium meliloti VO3128, heat shock, biological rep2 |
| GSM883381 |
Sinorhizobium meliloti VO3128, heat shock, biological rep3 |
| GSM883382 |
Sinorhizobium meliloti AB3, heat shock, biological rep1 |
| GSM883383 |
Sinorhizobium meliloti AB3, heat shock, biological rep2 |
| GSM883384 |
Sinorhizobium meliloti AB3, heat shock, biological rep3 |
| GSM883385 |
Sinorhizobium meliloti AB9, heat shock, biological rep1 |
| GSM883386 |
Sinorhizobium meliloti AB9, heat shock, biological rep2 |
| GSM883387 |
Sinorhizobium meliloti AB9, heat shock, biological rep3 |
| GSM883388 |
Sinorhizobium meliloti Rm1021, stationary phase growth, biological rep1 |
| GSM883389 |
Sinorhizobium meliloti Rm1021, stationary phase growth, biological rep2 |
| GSM883390 |
Sinorhizobium meliloti Rm1021, stationary phase growth, biological rep3 |
| GSM883391 |
Sinorhizobium meliloti VO3128, stationary phase growth, biological rep1 |
| GSM883392 |
Sinorhizobium meliloti VO3128, stationary phase growth, biological rep2 |
| GSM883393 |
Sinorhizobium meliloti VO3128, stationary phase growth, biological rep3 |
| GSM883394 |
Sinorhizobium meliloti AB3, stationary phase growth, biological rep1 |
| GSM883395 |
Sinorhizobium meliloti AB3, stationary phase growth, biological rep2 |
| GSM883396 |
Sinorhizobium meliloti AB3, stationary phase growth, biological rep3 |
| GSM883397 |
Sinorhizobium meliloti AB9, stationary phase growth, biological rep1 |
| GSM883398 |
Sinorhizobium meliloti AB9, stationary phase growth, biological rep2 |
| GSM883399 |
Sinorhizobium meliloti AB9, stationary phase growth, biological rep3 |
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| Relations |
| BioProject |
PRJNA151659 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36186_RAW.tar |
64.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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