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| Status |
Public on Mar 15, 2012 |
| Title |
Discrete molecular states in the brain accompany changing responses to a vocal signal. |
| Organism |
Taeniopygia guttata |
| Experiment type |
Expression profiling by array
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| Summary |
New experiences can trigger changes in gene expression in the brain. To understand this phenomenon better, we studied zebra finches hearing playbacks of birdsong. Earlier research had shown that initial playbacks of a novel song transiently increase the ZENK (ZIF-268, EGR1, NGFIA, KROX-24) mRNA in the auditory forebrain, but the response selectively habituates after repetition of the stimulus. Here, using DNA microarray analysis, we show that novel song exposure induces rapid changes in thousands of RNAs, with even more RNAs decreasing than increasing. Habituation training leads to the emer- gence of a different gene expression profile a day later, accompanied by loss of essentially all of the rapid "novel" molecular responses. The novel molecular profile is characterized by increases in genes involved in transcription and RNA processing and decreases in ion channels and putative noncoding RNAs. The ‘‘habituated’’ profile is dominated by changes in genes for mitochondrial proteins. A parallel proteomic analysis [2-dimensional difference gel electrophoresis (2D-DIGE) and sequencing by mass spectrometry] also detected changes in mito- chondrial proteins, and direct enzyme assay demonstrated changes in both complexes I and IV in the habituated state. Thus a natural experience, in this case hearing the sound of birdsong, can lead to major shifts in energetics and macromolecular metabolism in higher centers in the brain.
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| Overall design |
Adult male zebra finches were acoustically isolated and exposed to silence, novel song, or familiar song (exposure to testing song for 3 hours on the day prior to testing) on test day. The auditory lobule (AL) was collected 30 minutes after the onset of the testing experience. All samples were hybridized against the universal SoNG reference RNA pool, 6 biological replicates per group in each of 3 groups.
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| Contributor(s) |
Dong S, Replogle KL, Hasadsri L, Imai BS, Yau PM, Rodriguez-Zas S, Southey BR, Sweedler JV, Clayton DF |
| Citation(s) |
19541599 |
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| Submission date |
Mar 05, 2012 |
| Last update date |
Aug 18, 2014 |
| Contact name |
Kirstin Replogle |
| E-mail(s) |
replogle@igb.uiuc.edu
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| Organization name |
University of Illinois
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| Street address |
1206 W Gregory Drive
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| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL9554 |
Songbird Neurogenomics Initiative 20K Array |
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| Samples (18)
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| GSM885649 |
AL of adult male familiar song replicate 1 |
| GSM885650 |
AL of adult novel song replicate 1 |
| GSM885651 |
AL of adult silence control replicate 1 |
| GSM885652 |
AL of adult male familiar song replicate 2 |
| GSM885653 |
AL of adult novel song replicate 2 |
| GSM885654 |
AL of adult silence control replicate 2 |
| GSM885655 |
AL of adult male familiar song replicate 3 |
| GSM885656 |
AL of adult novel song replicate 3 |
| GSM885657 |
AL of adult silence control replicate 3 |
| GSM885658 |
AL of adult male familiar song replicate 4 |
| GSM885659 |
AL of adult novel song replicate 4 |
| GSM885660 |
AL of adult silence control replicate 4 |
| GSM885661 |
AL of adult male familiar song replicate 5 |
| GSM885662 |
AL of adult novel song replicate 5 |
| GSM885663 |
AL of adult silence control replicate 5 |
| GSM885664 |
AL of adult male familiar song replicate 6 |
| GSM885665 |
AL of adult novel song replicate 6 |
| GSM885666 |
AL of adult silence control replicate 6 |
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| Relations |
| BioProject |
PRJNA153099 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36269_RAW.tar |
33.6 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data not provided for this record |
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