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Series GSE36362 Query DataSets for GSE36362
Status Public on Jun 01, 2012
Title Transcriptional profiling of turkey muscle satellite cells transfected with MGP-siRNA during proliferation and differentiation
Organism Meleagris gallopavo
Experiment type Expression profiling by array
Summary In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene Matrix Gla Protein (MGP) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The MGP gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). Gene expression changes were investigated in satellite cells after 72 h of proliferation and after 48 h of differentiation. We identified novel candidate genes and pathways as playing potentially crucial roles in the MGP-mediated effects on the normal processes of proliferation and differentiation in turkey satellite cells previously identified by Velleman et al. (submitted).
 
Overall design This experiment was designed to investigate the role of MGP expression in turkey satellite cells during two crucial processes in muscle development: proliferation and differentiation. Satellite cells were isolated from 7-week-old turkeys from the RBC2 line. Cells were transfected with either MGP siRNA or a lipofectamine control; MGP expression was knocked down by over 50% with siRNA transfection (Velleman et al., submitted). Satellite cells were then induced to proliferate for 72h or differentiate for 48h; culture plates from each stage (n=4) were frozen until RNA extraction. Microarrays directly compared the MGP -knockdown to control from each of 4 culture plates and utilized a dye swap to equal 8 arrays for each of the cell developmental stages, proliferation and differentiation, and 16 arrays for the overall experiment investigating the role of MGP expression in satellite cell development and function. Hybridizations were performed in random order.
 
Contributor(s) Sporer KR, Velleman SG, Tempelman RJ, Strasburg GM
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Submission date Mar 08, 2012
Last update date Jun 02, 2012
Contact name Kelly R Sporer
E-mail(s) buckhamk@msu.edu
Phone 517-355-8474
Fax 517-432-0753
Organization name Michigan State University
Department Food Science and Human Nutrition
Lab Muscle Biology and Genetics Research
Street address 3365 Anthony Hall
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
 
Platforms (1)
GPL9788 Turkey skeletal muscle long oligonucleotide (TSKMLO) microarray
Samples (16)
GSM889644 MGP-knockdown vs Control (72h Proliferation) Replicate 1
GSM889645 MGP-knockdown vs Control (72h Proliferation) Replicate 2
GSM889646 MGP-knockdown vs Control (72h Proliferation) Replicate 3
Relations
BioProject PRJNA153331

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36362_RAW.tar 23.0 Mb (http)(custom) TAR (of CSV, GPR)
Processed data included within Sample table
Processed data provided as supplementary file
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